The Clinical And Experimental Research Of Simotang Treatment For Slow Transit Constipation

The first part Clinical research of Simotang treatment of slow transit constipation.Objective:Simotang is the commonly used clinical prescriptions for the treatment of slow transit constipation, In this papers we compare the clinical efficacy with oral lactulose for further studies.Methods:We randomly divided 64 cases of patients with slow transit constipation of The First Affiliated Hospital of Guangxi Medical University outpatients into two groups from 1 March 2011 to June 2014, according to generate a random number table SPSS 16.0 statistical package. One is the Simotang oral solution group of 32 patients,12 males and 20 females; the other is lactulose oral solution group of 32 patients,11 males and 21 females. There were no statistically significant differences between the two groups of gender, age, disease duration, symptom score before treatment, and severity of disease(P>0.05),and the two groups were comparable. The Simotang group was given Simotang Oral solution 1,20ml/times, three times a day, and lactulose group was given oral lactulose solution,10ml/times, three times a day.Seven days is a course of treatment. After two courses comparing the results of the efficacy of the two groups, TCM symptom score, clinical relapse rate and colonic transit time we analyzed the clinical efficacy.Results:The total effective rate of Simotang group was 92.85%, the total effective rate of Lactulose group was 73.08%. There were significant differences in the statistical analysis between the 2 groups comparing the overall clinical efficacy (P<0.05). After a course of treatment, compared the two groups the symptom score decreased significantly, but there was no statistically significant difference relative symptom score (P>0.05), After two courses of treatment, there was a significant difference between the two groups (P<0.05). Three months after the end of treatment follow-up Simotang relapse rate was 23.08%, lactulose group relapse rate was 47.37%, and there were significant differences between the two groups(P<0.05). before treatment, the colonic transit time of the two groups of patients showed no significant after statistical analysis(P>0.05).The difference was significant after treatment of the same group compared with pretreatment(P<0.05),and Simotang group compared with lactulose group, there was no significant difference in the impact of the ascending colon CTT values of the two groups after treatment(P>0.05), but there was significant different affect of CTT between the two groups according to descending colon, sigmoid, rectum and the entire colon(P<0.05).Conclusions:The effective rate of Simotang oral solution was more efficient than oral lactulose with respect to slow transit constipation. Two groups both had no significant adverse reactions. The recurrence rate of Simotang group was less than lactulose oral solution group stopping the medication. Simotang oral solution can significantly shorten CTT values of the descending colon, sigmoid colon, rectum and colon. It solved the problem of slow colonic transit from the etiologyThe second part Exploring the mechanisms of Simotang for treatment of constipation from c-kit/SCF gene expression and neural pathways.Objective:Exploring the mechanisms of Simotang for treatment of constipation from c-kit/SCF gene expression and neural pathways.Methods:The 120 healthy Wistar rats (Male and female are equal) weighing from 100g to 120g of were randomly divided into normal control group 30, the blank group 30, the placebo group 30 and the treated group 30. All rats were divided equally caged. The environment was maintained suitable. Normal control group was fed with ordinary dry diet, unlimited water. he model group 90 rats were fed diets containing compound diphenoxylate feed,and the dose was 8 mg/kg. d.120 days after feeding We determined the success of established rat model by determination of intestinal transport function by activated carbon gavage, then our successful model rats were randomly average divided into control group% the placebo group and the treatment group.Each group of rats were respectively measured intestinal transport function, colon EMG measurement, colon neuropathology detection, Real time PCR analysis after treatment.Results:Compound diphenoxylate can significantly inhibit defecation, make the intestinal contents delayed, and result in defecation decreased or difficult defecation in rats. Colonic myoelectric slow wave frequency of control group and Saline model group rats decreased, amplitude increased the coefficient of variation of slow wave frequency and amplitude was significantly higher than that of normal group and treatment group. Number of intramural ganglion significantly reduced and significant fibrosis in model group and saline control group. Relative contents of c-kit mRNA, SCF mRNA of model group and saline control group were significantly reduced.Conclusions:Simotang can significantly suppress the influence of drugs on modeling slow transit constipation rats, such as slowing colonic myoelectric slow wave frequency, increasing the amplitude and the frequency and amplitude variation coefficient, reducing the number of ganglia and fibrosis and by increasing the c-kit mRNA relative content, SCF mRNA relative content to treat slow transit constipation.