The Correlation Research On Tfh,IL-21 And Other Factors With Producing Autoantibodies In Immune Infertility

BackgroundInfertility is a specific type of reproductive problem, its etiology is complex. It has theoretical and social significance to research the infertility and find the foundation of its diagnosis and treatment. Immunological factors account for 10% to 20% in infertility cases. There are many autoantibodies generated in immunologically infertile patients. Studies have confirmed that Tfh could promote B-cell class switching and produced many autoantibodies, while its dysfunction could cause the autoimmune diseases. From these data we suspect that the production of autoantibodies is associated with Tfh and IL-21 in immunologically infertile patients.Objective:To provide a new diagnostic and treatment approach in immune infertility by studying the correlation of T lymphocyte subsets, cytokines, hormones and microelements with the generation of autoantibodies in peripheral blood of infertility patients.Methods:1. T cell subsets detection (FCM)2. Cytokine detection (ELISA)3. Hormones detection (ELISA)4. Microelements detection (AAS)5. Autoantibody detection and Correlation Analysis (ELISA)6. Statistical methodsResults:1. The expression of T cell subsetsPeripheral blood Tfh, accounting for the proportion of CD4+T cells in immunologically infertilite patients, was significantly higher than normal individuals (P<0.01).2. Evaluation of cytokines in serumCompared, with the control group, serum IL-2 and IL-4, IL-6, IL-21, TNFa and IFNy content was significantly higher in infertility patients(P< 0.01). The IL-8 increasing was not obviously, but was significant(P< 0.05). The other cytokines in serum showed no obviously changed.3. Evaluation of hormones in serumCompared with the control group, serum FSH、LH and E2 content was significantly higher in infertility patients(P< 0.01). But PRL、P and T showed no obviously changed.4. Evaluation of microelements in serumCompared with the control group, the content of Ca and Fe in serum was significantly decrease in infertility patients, but was not significant (P> 0.05). Zn and Cu were significantly higher in infertility patients (P< 0.01).5. Results from patients’serum antibody detection tests and Analysised the correlation of associated factors with autoantibodies.(1) AhCGAb, AsAb, AoAb, and EmAb in the infertility group were significantly higher than those in the control group (P< 0.01).(2) Tfh and IL-21 were positively correlated with the generation of AhCGAb, AsAb, AoAb and EmAb. IL-12 was positively correlated with the generation of AhCGAb and AsAb. TNFoc was negatively correlated with AoAb and EmAb production.(3) FSH and LH were positively correlated with the generation of AhCGAb, AsAb, AoAb and EmAb; But E2, PRL, P and T were not positively correlated with the generation of AhCGAb, AsAb, AoAb and EmAb.(4) Cu were positively correlated with the generation of AhCGAb, AsAb, AoAb and EmAb; But Ca, Fe, and Zn were not positively correlated with the generation of AhCGAb, AsAb, AoAb and EmAb.Conclusion:1、AhCGAb, AsAb, AoAb, and EmAb were significantly raised up in the infertility.2、The content of T cell subsets were changed in the infertility, Tfh and IL-21 were positively correlated with the generation of AhCGAb, AsAb, AoAb and EmAb.3、The content of cytokines in serum were changed in the infertility, IL-21 were positively correlated with the generation of AhCGAb, AsAb, AoAb and EmAb. IL-12 was positively correlated with the generation of AhCGAb and AsAb. TNFa was negatively correlated with AoAb and EmAb production.4、The content of hormones in serum were changed in the infertility, FSH and LH were positively correlated with the generation of AhCGAb, AsAb, AoAb and EmAb; But E2, PRL, P and T were not positively correlated with the generation of AhCGAb, AsAb, AoAb and EmAb.5、The content of microelements in serum were changed in the infertility, Cu were positively correlated with the generation of AhCGAb, AsAb, AoAb and EmAb; But Ca, Fe, and Zn were not positively correlated with the generation of AhCGAb, AsAb, AoAb and EmAb.6、The researches have shown that the autoantibodies manifested in immunologically infertile patients strongly correlates with Tfh, cytokines, hormones and microelements. These may provide new diagnostic and therapy indicators to screen for immunologic infertility.