Study On The Speciation Of Microelement And Biological Activities Of Clove

In recent years the research of microelements in Chinese traditional medicines has attracted more and more attention. Microelements are related to people's healthy, upgrowth and malady's prevention and cure closely. The research has been discovered that the pharmic effect of Chinese traditional medicines has the relationship with the kind and content of differently microelement. So determining the content and ratio of microelement in Chinese plant can provide enough information and academic basis for clarifying Chinese plant's action mechanism, discussing Chinese medicine's confection technics. The contents, primary and secondary-level speciation of microelements (Cu , Zn , Fe , Mn , Ni) in medical and edible dual purpose plant -clove, and biological activities of clove ,were studied detailed in this paper for the first time.The contents of some microelements in clove were determined by atomic absorption spectrometry, the relationship between the content and their effect was discussed. The study shows that the contents of Fe, Mn , Zn are abundant in clove, especially the contents of Fe and Mn are at best, and the contents of Cu and Ni are little. The microelements of Fe and Mn are the main resaons of diversified officinal value in clove and play an important role in the inner of the body, because the microelements of Fe and Mn not only have active role to the enzyme in the inner of the body, but also have the direct or indirect role to the nucleic acid and protein's anabolism, immunity and so on. The average Zn/Cu ratio of clove is lower than the ratio in serum or erythrocyte of valetudinarian of hypertension. Thus the clove can reduce the Zn/Cu ratio of erythrocyte in valetudinarian of hypertension, consequently reduce the hypertension of valetudinarian. The results offered some academic reference in selecting medicine and analyzing the speciation of microelement.Primary speciation of four elements (Cu, Zn, Fe, Mn) in clove were studied onthe basis of contents of microelements. The contents of primary speciation sample which had obtained from primary analytical flow were determined by atomic absorption spectrometry. The parameter of primary speciation analysis, such as extractive rate, immerse-residue ratio and so on, was also calculated. The concentration of four elements in residue of clove was high, which showed that the residue was still valuable. In the experiment of speciation analysis, we choose the microwave extraction way as the best extraction means at last by making contrastive experiment of different extraction methods.The research indicates that the immerse-residue ratio of Mn is best and Mn plays an important medical role, so Mn can be considered as the best characteristic element in clove. More datum and useful information were gained by primary speciation analysis than by total content of microelements. Therefore, the result of primary speciation analysis offered some academic reference for using Chinese herbal medicine, conforming analytical methods of higher speciation and exploring reasons of curing disease about Chinese herbal medicine.Secondary-level speciation of Cu. Zn, Fe and Mn in clove was studied on the base of the analysis of primary speciation. The organic and inorganic forms were divided by AmberliteXAD-2 macro reticular resin, Stable and labile forms were divided by 401 chelate resin. The presented result of secondary-level speciation indicated Cu, Zn, Fe and Mn all existed in jumbly forms, thereinto Mn , Cu, Zn almost existed in inorganic forms, Fe had half part organic and inorganic. So Cu, Zn , Fe and Mn almost existed in inorganic and labile forms in clove solution.Moreover, the result of experiment of antioxidable, antibacterial and anticancer role in clove indicated : The decay and deterioration of food can be reduced by clove as the natural antioxidant because of it's strong inhibiting role to POD's activity. The clove has strong antibacterial role to the Eschrichia coli and Staphylococcu Saureus, especially the antibacterial ratio to Staphylococcu Saureus can achieve 100%. It is strongly proves that the clove is safe and credible as natural antiseptic, and can be used for the food and cosmetic's antisepsis. The clove water immersion original fluid has the very strong elimination function to NO2", the elimination rate of clove sample fluid to the NO2" can achieve 100%, so the clove as the natural food additive can stop the inferior nitre base chemical compound's synthesis under the certain condition and has the remarkably anti-cancerous role. It can make beneficial attempt for expanding medicine source, enhancing the pharmacological action and expanding applicability if combine the chemical compound of clove with the pharmacological action organically. It also establishes the base for the natural medicine's distilling, decorating andempoldering.