Clinical Research And Experimental Research On Treatment Of Primary Hapatic Carcinoma By Jiaweisini Prescription

ObjectiveIn this study, JWSNP plus detoxification, expectorant dampness medicine, the use of H22and S180tumor model of mice, using cytology and immunohistochemistry technology research, comparison of observed JWSNSP different concentrations of anti-tumor effects and effects on immune function, explore the mechanism of action of anti-hepatoma JWSNP. Meanwhile, the clinical efficacy JWSNP treatment of advanced primary hapatic carcinoma.MethodAccording to inclusion and exclusion criteria collect used JWSNP therapy in patients with advanced primary hapatic carcinoma, liver cancer using quality of life scale (QOL-Lc2.0), the patient’s general information gathering, live acts, four diagnostic syndrome type and laboratory parameters, using the statistical package EpiData3.0establish a database for data entry and logical proof data entry errors discovered immediately corrected under observation table, to ensure complete and accurate data, export data proofread and correct as SPSS files; using SPSS17.0analysis software, survival time, according LifeTables life table method and survival curves representation; tumor changes in solid tumors by UICC evaluation criteria to determine efficacy.Established solid H22tumor model of mice were fed high, medium and low concentrations JWSNP decoction (50,25,12.5g/kg/d), intraperitoneal injection of5-fluorouracil ah steep (5-FU)(20mg/kg/d) positive control; solid tumor model after administration8d detect the tumor weight, weight, spleen index and thymus index, MTT assay of each group of natural killer (NK) cells and T lymphocytes live cell proliferation wins, ELISA assay activity of serum interleukin-2(IL-2) is, TNF-α and VEGF levels, immunohistochemical method to detect tumor microvessel density (MVD); ascites tumor model ting model group died administration ended, survival time was observed in each group.Established solid tumor models S180tumor model of mice, gavage JWSNP dose (25g/kg/d), for10consecutive days, the determination of tumor weight of each group.H22tumor model of mice quality of life analysis using statistical software SPSS17.0Kaplan-Meier method. Other data processing method using One-Way ANOVA.ResultClinical research results:A total of35cases of primary hapatic carcinoma patients, including15cases of stage III patients (43%),20cases (57%) IV stage patients. After JWSNP treatment, patients longest survival time1085days, the shortest survival time of39days. Where half the survival rate of40%(14people), one-year survival rate of26%(9),2-year survival rate of14.2%(5),3-year survival rate of2.8%(1), the median survival of5.5months, who has survived a number of cases. Tip JWSNP primary hapatic carcinoma patients on prolonged median survival time and survival of a certain effect. None of the patients in CR35patients, four cases (11.4%) PR,17例(48.6%) SD,14例(40%) PD, tips JWSNP control in advanced primary liver tumor have some effect。After4weeks of treatment, compared with before treatment after8weeks of treatment, patients in physical function latitude, the latitude mental function, functional latitude symptoms, social function and QOL-LC latitude total score, etc. have increased (all P<0.05), tip after JWSNP treat patients and compared the physical, psychological symptoms, social functioning and overall quality of life before treatment has significantly improved.8weeks after treatment and after4weeks of treatment, patients with reduced physical function score latitude areas (P<0.05), mental function in latitude, the latitude was no difference in symptom function, social function and QOL-LC latitude total score, etc.(all P>0.05), suggesting Jiaweisinisan efficacy of long-term improvement in physical function in patients with less than ideal, but in psychological symptoms, social functioning and overall quality of life, the improvement lasting effect. After4weeks of treatment, compared with before treatment after8weeks of treatment, liver function Child-Pugh score decreased (all P<0.05), prompted JWSNP may improve liver function in patients.8weeks after treatment and after4weeks of treatment, Child-Pugh score increased (P<0.05), decreased liver function, suggesting JWSNP improve liver function in long-term effect is not ideal.Experimental research results:①JWSNP prolonging survival time of tumor-bearing mice have a role, especially high-dose JWSNP can significantly improve survival time.②JWSNP significant role in tumor-bearing mice increased body weight, especially JWSNS dose group and low dose.③JWSNP with increasing dose has some inhibitory effect; on H22tumor model of mice, high-dose inhibition JWSNP rate18.6%(P<0.05); liver cancer in mice on S180tumor model of mice, high dose rate JWSNP inhibition19.9%,12.9%(P<0.05).④JWSNP high and low dose group was inoculated with H22tumor model of mice thymocytes stimulated increased (P<0.05); inoculated with S180tumor model of mice, spleen index Jiaweisinisan increase in tumor-bearing mice (P <0.05), the5-FU treatment in mice caused atrophy of the spleen and chest were significantly (P<0.01); while JWSNP significantly increased the activity of IL-2(P<0.05), improved tumor-bearing mice T lymphocyte activity (P<0.05), NK cell proliferation (P<0.05), prompted JWSNP its anti-tumor effect may improve immune function related.⑤JWSNP can significantly reduce tumor model of mice serum VEGF concentrations (P<0.05), was observed in tumor tissue stained karyopyknosis significantly reduce the number of blood vessels, suggesting JWSNP inhibit tumor tissue angiogenic effect. In addition, the role of tumor tissue JWSNP qualitative change between cancer cells and more cell-cell adhesion enhancement, suggesting that it can inhibit tumor invasion and metastasis.⑥JWSNP tumor model of mice can reduce serum TNF-α concentrations (P <0.05), which may be a protective mechanism of liver function.Conclusion①JWSNP to prolong survival in patients with primary hapatic carcinoma, the median survival time and control the tumor has some effect, but the effect is not ideal; which can significantly improve the quality of life of patients in the short-term (4weeks), improve the patient’s bed symptoms, improve liver function; in the long term (8weeks) to maintain a good quality of life in the state, but because of critically ill patients with advanced liver cancer, liver failure, simply use the long-term efficacy JWSNP difficult to maintain and improve liver function.②JWSNP prolong the survival time of tumor model of mice and increased body weight in mice; JWSNP high dose of H22, S180liver cancer tumor model of mice had a higher inhibition rate; JWSNP can increase tumor-bearing small mouse immune function, which may be one of the mechanisms of resistance to liver cancer; while JWSNP reduce TNF-α concentrations, may be a protective mechanism of liver function; JWSNP inhibit tumor angiogenesis, increased cell and adhesion between cells, suggesting that it can inhibit tumor invasion and metastasis.