| Parkinson’s disease is a progressive degenerative disorder of the central nervous system. The treatment of the disease at present regardless of medication or operation treatment, can improve the symptoms, development cannot prevent the disease, can not cure. Surgical treatment of costs and adapt to the limitations of non-motor side effects, drug treatment of complications and symptoms of dyskinesia fluctuation movement and medicine and disease itself brings is the treatment of PD problems. Traditional Chinese medicine has little toxic side effect and through the dialectical theory of governance, regulating the balance of yin and Yang from the overall characteristics of the patients, further research of trad it ional Chinese medicine in the treatment of Parkinson’s disease has important significance of advantage.This study by observing the differences before and after treatment in patients with Parkinson disease symptoms and non-motor symptoms of western scale score change and Parkinson’s disease with constipation of functional constipation in patients with CCK, Ghrelin and other parts of brain gut peptide secretion level of. At the same time, based on the classic MPTP model made by rotenone, copy the model of Parkinson’s disease, the pathogenic process simulation "chronic low dose administered orally to the enteric nervous system, dorsal vagal nucleus, substantia nigra intermediolateral nucleus such as multi-body system of α-synuclein accumulation", from mass spectrometry, radioimmunoassay and observation on the level of brain enteric nervous tissue, partial neurotransmitter content and serum ghrelin level, at the same time with Chinese traditional medicine as a means of intervention, of compound peibuganshen effects on the animal model of cerebral, enteric neurons, clear the mechanism of action and the link.The clinical research[Objective] to explore effect of TCM treatment of Parkinson’s disease and motor symptoms non-motor symptoms, in order to provide objective basis for exploring the advantages of TCM treatment of Parkinson’s disease.[Methods] the Hoehn-Yahr grading standard, stratified randomization,60cases of Parkinson’s disease patients were randomly divided into treatment group and control group, two groups based on the original PD western medicine treatment, the treatment group was treated with compound peibuganshen treatment, the relevant comparison before and after treatment of TCM symptom score scale and Parkinson’s disease symptoms, non-motor symptoms, curative effect of compound peibuganshen treatment of Parkinson’s disease. At the same time,12cases of const ipat ion in pat ient s with Parkinson’s disease, serum CCK Ghrelin levels were measured, compared with patients with functional constipation, influence the pathogenesis of preliminary inquiry of different diseases on the secretion of some brain gut peptide.[results]1, non-motor symptoms of Parkinson’s disease and can appear in any one stage of the disease, part appeared earlier than before motor symptoms, sleep disorders, constipation, loss of sense of smell, sensory disturbance and sexual dysfunction in motion before symptoms appear.2, compound peibuganshen can significantly improve the patient’s motor and non-motor symptoms, non-motor symptoms of fatigue, constipation, drowsiness symptoms improved obviously.3, Parkinson’s disease with constipation in ghrelin secretion, and no correlation between ghrelin secretion levels with severity. Parkinson’s disease with constipation and functional constipation in patients with different pathogenesis, there are differences in Ghrelin secretion level.[Conclusion] compound peibuganshen can significantly improve Parkinson’s disease, Parkinson’s disease with constipation and effective on the treatment of non-motor symptoms of fatigue.The experimental study1. compound peibuganshen effect on PD mice behavior and morphology[Objective] through observation and brain pathology in PD mice behavior, effect of preliminary inquiry model of mice behavior, morphology and the influence of the TCM treatment of PD. Research of traditional Chinese medicine, treatment of PD motor symptoms and pathological changes of[method] Based on MPTP modeling, the reference of foreign literature,"PD pathogenic process of chronic low dose administered orally to the enteric nervous system, dorsal vagal nucleus, substantia nigra intermediolateral nucleus such as multi-body system of α-synuclein accumulation" to rotenone, determination of the Quartet from the pole test, test, suspension swimming test, autonomic activity in mice behavior were observed, and through HE staining and immunohistochemical methods on brain morphology observation, to explore Chinese medicine in the treatment of PD symptoms and pathological changes of[results]1, rotenone group mice model with simulated PD "pathogenesis of disease development.".2, RT and MPTP in mice model can lead to behavioral changes, mainly for the slow movement, reduced activity, reaction ability to slow down. Given the Chinese herbs treatment or anti PD medicine Madopar treatment can improve.3, the model group mice brain nerve cell degeneration, mainly in the substantia nigra, Chinese herbs and Western medicine treatment can improve the resistance of PD modified parts of nerve cells, thereby improving the symptoms of PD.4, model of brain tissue in mice TH positive cells decreased, that reduce the content of DA in brain PD, giving the Chinese herbs and Western medicine in the treatment of anti PD can increase the content of DA effect from point to improve symptoms.[Conclusion] compound peibuganshen can improve the degeneration of nerve cells, and by increasing the DA content to improve motor symptoms.2, effects of compound peibuganshen on PD mice fecal water content and CCK, Ghrelin content[Objective]To explore the effect of compound peibuganshen on PD model of brain-gut peptide content, target clear influence brain gut peptide content in Parkinson’s disease with constipation and treated by Chinese medicine.[method] this study based on the classical MPTP model, in order to rotenone replication model of Parkinson’s disease, the pathogenic process simulation "chronic low dose administered oral ly to the enteric nervous system, dorsal vagal nucleus, substantia nigra intermediolateral nucleus such as multi-body system of α-synuclein accumulation", from the feces of water and plasma gastrointestinal dysfunction of PD mice by CCK, the content of Ghrelin, at the same time with Chinese traditional medicine as a means of intervention, to observe the effect of compound peibuganshen on the animal model of brain gut peptide content[results]1, RT and MPTP after modeling the mice constipation symptoms, give the traditional Chinese medicine compound peibuganshen treatment or anti PD medicine Madopar treatment can improve.2, RT group mice in blank group, Chinese medicine group, western medicine group CCK levels compared with the model group has difference, have differences between MPTP mice CCK traditional Chinese medicine group and the model group and blank group, associated with the pathogenesis of CCK PD constipation secretion, and compound peibuganshen by adjusting the CCK bleeding flat to improve the symptoms of constipation.3mice in MPTP model group, plasma CCK levels and brain DA content was positively related to Parkinson’s disease, considering the nigrostriatal DA neurons lost too much at the same time, a part of the brain-gut double regulating gastrointestinal hormones such as CCK secretion also may be affected, resulting in PD mice intestinal dysfunction.4, RT group and MPTP group were observed between Ghrelin values were not significantly different, and the level of plasma Ghrelin and DA content in cerebral no correlation.[conclusion]PD in constipation model mice, may be associated with Parkinson’s disease nigrostriatal DA neurons lost too much at the same time, CCK secretion is affected by lead to constipation, compound peibuganshen by regulating CCK secretion levels to improve the symptoms of constipation.3, compound peibuganshen effect on PD mouse striatum DA, HVA,5-HT, Ach content[Objective] by determining the content of different interventions and in brain tissue of the rats with DA, Ach,5-HT, HVA, explore the effect of PD effects on neurotransmitters part of the nerve and the treatment of Chinese medicine.[Method] in this study, RT and MPTP model, the PD pathology and neurobiochemical changes from the pathogenesis of PD simulation, because the tissue samples containing various interfering substances, the content of DA, Ach,5-HT, HVA measured liquid-liquid extraction and detection, concentration by liquid-phase tandem mass spectrometry method for the determination of DA,5-HT, Ach, HVA. The content of brain tissue was measured with different intervention methods of small rats, Ach,5-HT, DA, HVA, explore the effect of PD on neurotransmitters in effect part of the nerve and the treatment of Chinese medicine.[results]1, RT and MPTP model can be reduced to brain tissue DA and its metabolite HVA in PD, consistent with pathological changes of striatal DA neurotransmitter insufficient. In2, PD in early Ach and the changes of5-HT content than DA significantly, and Ach in the basal ganglia of change is not obvious, may also be related to PD stage, the late involvement of spirit, cognition, sleep disorder.3, pnlk and anti PD medicine beauty Duobake increased the content of DA and HVA in brain tissue, and the traditional Chinese medicine used mainly by increasing the content of HVA DA metabolite thus increasing DA content.4, it could increase the content of chloroform in the cerebral cortex of DA, but no difference between the effects on RT and MPTP mice in blank group DA and HVA content, does not affect the curative effect of the traditional Chinese medicine group and Western medicine group decision.[Conclusion]RT and MPTP can generate PD symptoms by reducing brain tissue DA and its metabolite HVA content, compound peibuganshen can increase HVA content of metabolites of DA and increase the content of DA. |
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