The Anti-Inflammatory And Antu-Parkinson’s Disease Effect Of FJ1and Its Underlying Mechanism

3β-angeloyloxy-8β,10β-dihydroxyeremophila-7(11)-en-12,8a-lactone is an activity eremophilenolide sesquiterpenes compound extracted from traditional Chinese medicine Farfugium japonicum. Farfugium japonicum named "Lian-Peng-Cao", a perennial herb that belongs to the Compositae family, Mainly distributed in China, Japan, southeast and South Korea. In China, it has been traditionally used in Chinese folk medicine for the treatment of sore throat, cold and cough and so on, suggesting that the active extracts with anti-inflammatory effect.Inflammation, a host defense mechanism, is an immediate response of the body to tissue injury caused by microbial infection and other noxious stimuli. However, inadequate resolution of inflammation and uncontrolled inflammatory reactions can evoke a state of chronic inflammation, which is a common etiologic factor for various human ailments including cancer. This subject is designed to verify FJ1validation activity and resistance to clarify its mechanism of action. We use Lipopolysaccharide (LPS) inducing the mononuclear macrophage in mice leukemia cells (RAW264.7) to build the Inflammatory cells model. Determined by MTT test FJ1cytotoxic activity as well as the protection of LPS damage cell activity, the results show that, both with time and dose dependent, cytotoxic within the scope of the security, FJ1can improve the cell activity of protection groups. Determination of NO discharge as the important indicators of inflammation by the nitric oxide kit, result display that pretreatment of RAW264.7cells with FJ1significantly inhibited LPS-induced NO production, And with dose dependent. Flow cytometry technology to detect the level of reactive oxygen species, and the results are in conformity with the above conclusion. Western blot investigates the protein expression level of ERK, p38, JNK, p-ERK, p-p38, p-JNK, NF-κB, IκB-α and p-IκB-α, it turned out that, the expression level of ERK, p38and JNK have no obvious changes, the protein expression level of p-ERK, p-p38, p-JNK, NF-κB and p-IKB-a reducding with increasing FJ1concentration, the protein expression level of IκB-α is opposite. RT-PCR test the expression of iNOS, COX-2and TNF-a, the results show that, all three were significantly reduced with the increase of concentration of FJ1. In conclusion, FJ1has significant protective activity on inflammation cell model and be a new contribution of traditional Chinese drug to the world’medicine.Parkinson’s disease (PD) is a common nervous system degenerative disease, its clinical manifestations include static tremor, bradykinesia, myotonia and posture gait disorder, at the same time it could be associated with depression, sleep disorder and constipation in patients with non motor symptoms, bring great pain to patients and their families. Parkinson’s disease is closely associated with oxidative stress, the antioxidant activity of FJ1shown in the investigate of anti-inflammatory activity suggests the potential activity of Parkinson’s disease resistance. Classic Parkinson’s cell model is made up of1-methyl-4-phenyl-5-four hydrogen pyridine (MPTP) induced sewer rat adrenal pheochromocytoma PC12cell lines, paraquat (PQ) is a common agricultural toxins, its structure nearly as same as the activity part of MPTP part MPP+, so in recent years many have been used to build the new model of Parkinson’s disease, this study adopts this kind of model, it’s the first time for our lab to build this model, it is necessary to detect some indicators. Determined by MTT test cytotoxic activity of PQ, the results show that the PQ inhibit the proliferation of PC12. both with time and dose dependent. Flow cytometry technique to determine cell mitochondrial membrane potential and cell apoptosis, it turns out that the high concentration of PQ decreased cell membrane potential and induced PC12apoptosis. The above results have been same as the reported data of MPTP and other laboratory to build the model. So it could be used as a following study experiment. In the protective activity research of FJ1on PC12cell, determined by MTT test the affect of FJ1on PQ induced PC12cell, the results show that FJ1could protecte cells and with a time and dose dependent. Flow cytometry technology to detect cell active oxygen levels, mitochondrial membrane potential and cell apoptosis, it shows that FJ1could suppress the growth of ROS and apoptosis caused by PQ, raise the level of MMP. Western blot investigates the protein expression level of ERK, p38, JNK, p-ERK, p-p38, p-JNK, Bcl-2and Bax, it turned out that, the expression level of ERK, p38and JNK have no obvious changes, the protein expression level of p-ERK, p-p38, p-JNK, Bcl-2reducding with increasing FJ1concentration, the protein expression level of Bax is opposite.This paper studied the anti-inflammatory and anti-Parkinson’s disease extraction activity of the Farfugium japonicum (FJ1) in this lab, for the clinical treatment of inflammatory disease and neurodegenerative diseases such as Parkinson’s disease to provide experimental basis, in addition, Some studies indicate that, The wide application of Parkinson’s disease in recent years associated with the widely used of PQ, The environmental factors of Parkinson’s disease suggest the environmental and epidemiological significance of this research...