The Clinical And Experimental Study Of Depression Treatment By Jin’s Three Neddles Therapy

ObjectiveTo observe the effect and safety of depression treatment by Jin’s Three Needles and discuss the related mechanism;to provide more evidence for depression treatment by Jin’s Three Needles and to spread this method to clinic.MethodsThis study was divided into two aspects:clinical studies and animal experiments(1) clinical study60cases were stratified random into two groups.30cases were in Jin’ s Three Needles group and30cases were in Seroxat group.①the Jin’s Three Needles group:Acupoint selection:The Four-Shen Points,Naosanzhen, Brain tri-points (two sides), addition and subtraction with symptoms. Inserting needle According to the operation rules, reinforcing and reducing with different symptoms.30minutes each time, once per day with4weeks as a course.②the Seroxat group:Given seroxat20mg daily in the morning,4weeks as a course.After the end of treatment, collected and counted the data.(2)animal experimentsAfter1week adaptive feeding,220-250g SPF SD rats were randomly grouped into4groups:the normal group、the model group、the Jin’s Three Needles group、the Seroxat group,8rats each group. All these rats were under Chronic unpredictable mild stress (CUMS) and feeded alone to make the model of depression, except the normal group.The normal group were caught everyday without any treatment. the rats in the model group were accepted CUMS stimulation for1hour and caught with no treatment. One hour after CUMS, the rats in Jin’s three Needle group were accepted Jin’s three Needles therapy, one time a day, a total of18days; The rats in seroxat group were given paroxetine by intragastric administration,10mg/kg per day,18days as a course.ResultsThe clinic results:(1)Hamilton Depression Scale scoreAfter acupuncture treatment, the HAMD score of the Jin’s Three Needles group was significantly decreased, this meant the Jin’s Three Needles was effective, which was equal to the effect of the Seroxat group.(2)Efficacy resultsThe efficiency of two groups (cure+significant progress+progress/total) were100%. The differences between the two groups was no statistical significance.(3)TESS scoreTwo weeks after treatment, the side effects on Jin’s three needles group was less than seroxat group.(4)the level of DA, NE,5-HT in peripheral blood;Jin’s three needles can improve the level of DA, NE,5-HT in patients’ peripheral blood,but seroxat can not improve the level’s of DA, NE.Animal experiments results:(1)Sucrose consumption testOn the ninth day of CUMS, the rats in Jin’s three needles group and seroxat group were more interested than in the model group;On the18th day of CUMS, the rats in Jin’s three needles group and seroxat group were more interesting than in the model group, but the Jin’s three needles group’s rats were less interesting than in the seroxat group.(2)Open-Field experiments:On the end of CUMS, the rats’horizontal and vertical movement in Jin’s three needles group and seroxat group have improved more than in the model group, but the rats horizontal movement in seroxat group have improved more than in the Jin’s three needles group.(3)The level of DA,NE,5-HT in hippocampus tissue of rat brain:NE:After treatment, there was no difference beteew these three CUMS groups, and the NE level was lower than the normal rats.DA:Jin’s three needl os group increased significantly, whi le seroxat group did not.5-HT:both Jin’s three needles group and Seroxat group were increased significantly, and Seroxat group increased more than Jin’s three needles group.Conclusion(1)The depression treatment with Jin’s three needles is safety, effective and little side effect;(2)Jin’s three needles can improve the peripheral blood NE, DA,5-HT levels of the depressive patients;(3)Jin’s three needles can improve the depression model rats’interests and ameliorate status of depression;(4)Jin’s three needles can improve the level of DA and5-HT in the depression model rats’hippocampal tissue, while NE is not improved obviously.