Theoretical And Experimental Research On The Novel Technology Of Microscopic Configuration Based Surface Plasmon Resonance Biosensors

Surface Plasmon Resonance (SPR) biosensing technology has many advantages such as label-free, high sensitivity and real-time dynamic detection, it has been wide applications in Biomedical and Chemical fields especially in proteomics research and drug discovery. Since there is a steep phase change when SPR phenomenon occurs, SPR biosensors based on phase interrogation can provide much higher detection sensitivity compared with that based on intensity, angular or spectral interrogation, however with small detection dynamic range.Due to the character of mass-pass,thesurface plasmon resonance imaging based on the configuration of Kretschmann has been wide applications, but the imaging resolution is lower, which is affect by the propagation distance of surface plasmon resonance.Therefore, this paper proposes a new SPR biosensor system with axisymmetric vectoring beam differential interferometric based on the microscopic structure, not only to increase the dynamic range of the phase-type surface plasmon resonance sensor, and has a high sensitivity, high imaging resolution. The main contents include:First,we propose a novel type of mixing light beam mixed with any proportion of radially and angular polarized, used to excitation surface plasmon resonance under high number aperture microscopic structure.The two kinds of polarized light as a signal light and the reference light can measure SPR phase by differential interferometry, combine with angle measurement to achieve large dynamic range simultaneously.Second, we propose radial and angular four petal light beam to achieve strict common-path measurement,reduce the interference of environment noise; we also propose six phase stepping technology in phase extraction process to improve the measurement speed,to reduce the interference of light source and PZT noise. With these improvements,the system is further to enhance the sensitivity.Finally, we build a surface plasmon resonance sensor scan imaging system, which combines the use of microfluidic chips and, to achieve automatic control. We have performed measurements of the biological samples with a high-resolution, large dynamic range, high image resolution.