Protein@Cu_2-xO Composite Nanoparticles:the Properties Of Near-infrared Surface Plasmon Resonance And In-vivo Imaging

Due to their unique physical and chemical properties,near-infrared active materials show attractive application prospects in tumor diagnosis and treatment.In this paper,a novel near-infrared absorption nanoparticles,BSA@Cu2-xO NPs were obtained by simple one-step method.Because of their good photothermal conversion efficiency and photoacoustic imaging effect,they could be applied to photothermal therapy.Compared with other near-infrared active materials,BSA@Cu2-xO NPs has the following two advantages in tumor diagnosis and treatment:First,their absorption peak is located in the second biological window,where the biological matrix has little absorbent.So,the background signal interference can be well avoided.Second,they can be quickly cleared by kidney metabolism,thus,they should possess low biological toxicity.In detail,this dissertation has four chapters as the following:In the first chapter,we summarized the developments of near-infrared active materials in recent years.In this section,the advantages and disadvantages of near-infrared plasma materials for tumor diagnosis and treatment were demonstrated.Finally,we proposed the issues to be solved urgently for near-infrared plasma materials and introduced the idea of our work.In the second chapter,we displayed the preparation,structure and near-infrared surface plasmon resonance properties of BSA@Cu2-xO NPs.Firstly,we first discovered a novel near-infrared plasma material(BSA@Cu2-xO NPs)under a gentle reaction condition(55?),which had a strong absorption in the near-infrared region(1300 nm).Secondly,the optimized experimental conditions were studied.Furthermore,the physicochemical performances of BSA@Cu2-xO NPs,obtained under the optimal synthesis conditions,were demonstrated.Finally,the universality of our method was studied.In the third chapter,we explored the bioapplications of the BSA@Cu2-xO NPs in vitro and in vivo.First,the NPs have a large extinction coefficient(8.20 L g-1 cm-1)at 808 nm excitation,showing good photothermal conversion efficiency(47.7%).When applied to cell photothermal erosion,they have a good photothermal erosion effect.Secondly,the NPs have a good photoacoustic imaging effect,successfully used for tumor photoacoustic imaging.Third,the NPs can be rapidly cleared by kidney metabolism.Finally,the NPs also have some fascinating properties,including low biotoxicity,high specificity and good photothermal stability.The last chapter highlighted the innovation of our work and prospected the potential bioapplications.