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Preparation Of Nanowire Array And Composite Films Based On Carbon Nanotubes For The Construction Of Electrochemical Biosensors

Posted on:2008-12-25Degree:DoctorType:Dissertation
Country:ChinaCandidate:M H YangFull Text:PDF
GTID:1118360212975603Subject:Analytical Chemistry
Abstract/Summary:PDF Full Text Request
There has been considerable interest in studying biosensors because they have potential applications in medical treatment, medicine, bioengineering, environmental protection and food industry. Recently, diabetes, as one of the main diseases that threaten human health, suffers more and more people, and is the third dangerous disease following cardiology and cancer. Glucose biosensors can be used to determine concentration of glucose in the blood conveniently and with high efficiency, so that have widely used clinically. But the conventional glucose biosensors have many problems such as low sensitivity, slow response and poor stability. In the past few years, nanomaterials have been widely researched and used. Applying nanomaterials for the fabrication of biosensors will greatly improve the performance of the resulting biosensor. This research prepared many nanomaterials with different morphology, taking advantage of the good physical, chemical, electrocatalytic and biocompatibility of these nanomaterials and using them for the preparation of sensors. The modified sensors can be used to determine hydrogen peroxide in low potential with high sensitivity, ideal for the construction of biosening platform. Immobilizing oxidases such as glucose oxidase and cholesterol oxidases onto the platform with different method, the resulting biosensors have high sensitivity, low detection limit and fast response time. The details are summarized as follows:(1) Prepared nanowire and nanotube array using template method. The large surface area of these nanostructures can increase the amount of enzymes immobilized, at the same time provide a friendly microenvironment for enzyme loading. It would be a significant advancement if perpendicularly aligned nanowire and nanotube array could be formed as sensing materials. The well defined surface area facilitated enzyme-substrate contact, greatly improving the performance of the resulting biosensor. First, gold nanowire array can be grown by electrodeposition in polycarbonate membrane directly immobilized on glassy carbon electrode surface. Taking advantages of the well defined surface area of the nanowire array and biocompatibility of metal gold toward biomolecules, we selected hemoglobin and investigated the efficiency of protein immobilization on gold nanowire array and the direct electrochemistry of hemoglobin. Uv-visible and FT-IR spectroscopy indicated on obvious denaturation of the proteins was observed after adsorption onto the...
Keywords/Search Tags:Nanowire array, Template method, Carbon nanotube, Synergy effect, Biosensor, Enzyme, Glucose
PDF Full Text Request
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