Preparation Of Glucose Oxidase Electrode Based On The Short Of Multi-walled Carbon Nanotubes

With the aging of the population increasing and the development of people lifequality, the incidence of diabetes showed a trend of rising year by year, and becomethe second only to cancer and cardiovascular, its diagnosis and treatment has becomethe priority of the medical profession. Therefore, how to convenient, fast andaccurately detect the contents of blood sugar has become more and more important.In recent years, electrochemical biosensor because of the advantages of shortingresponse time, highing sensitivity, small volume, has been widely attention andapplication in environmental monitoring, biological, chemical, pharmaceutical andhealth care, etc. In the study of biological sensors, the third generation enzymebiosensor with the characteristics of the enzyme specificity and high selectivity anddetecting of complex samples directly, showing great application prospect. However,it also has many disadvantages, such as immobilized and hidden of active center andMaintained biological activity, etc., which greatly limits the development of enzymebiosensors.There is a new approach to studying further on the enzyme biosensor because ofthe emergence of new nano. Immobilized enzyme using can implement directlyelectron transfer between the electrode and active sites of the enzyme and keep goodmicroenvironment, that is due to nano materials have large specific surface area, themacroscopic quantum tunnel effect, high biocompatibility and good mechanicalproperties and electrical conductivity, so it is an indispensable link in thedevelopment of biosensors.This article was preliminary prepared four glucose oxidase electrode basing onthe materials itselves and the synergy between nanoparticles, then detected by CyclicVoltammetry Curve and Amperometry, the research findings show that the preparedworking electrode can implement directly electron transfer between the electrode andactive sites of the enzyme, as well as the good current response of glucose solution.Comparing performance parameters of four kinds enzyme electrodes, the enzyme electrode modified with short multi-walled carbon nanotubes and Nano-TiO2hascertain advantages, and its linear range of0.1-1.0mM, linear equation I（μA）=14.17C（glucose）+0.711, the linear correlation coefficient R=0.999, the responsesensitivity0.241mA·㎝-2·mM-2that is expected to provide reference for the furtherstudy of enzyme biosensors.