The Development Of Molecularly Imprinted Bulk Acoustic Wave Biomimetic Sensor And Protein With Anticancer Drug Action

Due to its outstanding advantages such as broad sensing-spectrum, high sensitivity and low cost, the theories and applications of bulk acoustic wave sensors have been developed rapidly during past ten years. Now, its applications have been extended to the fields of pharmaceutical analysis, biochemistiy, molecular biology, genic engineering, clinic immunology, etc.. In 21st century, miniaturization, imitation of biology and intelligence are the main goals of the sensor technology. The emergence of molecular imprinting technique will doubtlessly accelerate the development of biomimetic sensors. In post genie era, one of tasks is to understand the interaction between proteins and small molecules and looks for new drugs in genie level to improve the quality of human life. The main results of this work are summarized as follows:1.For the first time, biomimetic bulk acoustic wave (BAW) sensors based on the molecularly imprinted polymers (MIPs) were fabricated and applied to the determination of phenobarbital and pyrimethamine in organic and aqueous solution, respectively. The MIPs were synthesized using methacrylic acid as the functional monomer and ethylene glycol dimethacrylate as the crosslinker by the non-covalent method. The sensors exhibited good sensitivity, selectivity and long-time stability even in harsh chemical environments.2.The preparation of biomimetic bulk acoustic wave sensors for phenylalanine, atropine and pesticide 2,4-dichlorophenoxyacetic acid based on the electrosynthesized molecularly imprinted polymers using o-aminophenol, o-phenylenediamine as monomers is first proposed. The properties of these sensors and the kinetics of binding of template molecules to polymers are investigated in detail. This method not only resolves the problem of modification of polymers but also offers a possibility of miniaturization of sensors, which is one of the major goals of chemical sensor technology.3.The continuous molecularly imprinted polymer rod was prepared in situ by using L-phenylalanine derivative as template molecules, methacrylic acid as functional monomer, ethylene glycol dimethacrylate as crosslinker. The enantiomers of DL-phenylalanine were successfully separated using a seriespiezoelectric quartz crystal sensor as the detector. The selectivity factor a was 1.87 and the resolution R~ was 1.09. The effects of preparation of polymer, the composition of mobile phase and the flow-rate on the separation result were investigated in detail.4.For the first time, the real-time on-line studies of adsorption of human serum albumin on different surfaces and the interaction between human serum albumin and anticancer drug vepesid were carried out using combined electrochemical quartz crystal impedance with electrochemical impedance spectroscopy system. When human serum albumin was adsorbed on the surface of Au electrode or C12SH modified Au electrode, different conformations were resulted and the processes of binding vepesid were also different. The corresponding kinetic parameters were obtained by fitting the response curves. The values of heterogeneous standard charge transfer rate constant for Fe(CN)637 Fe(CN)64 were obtained from the cyclic voltanimograms and the electrochemical impedance spectroscopy, and the electric double layer capacitance was calculated from the impedance semicircle.