An Analysis Of The Inhibition Of Tangzukang Compound Capsule On DF And Its Mechanism

Diabetic foot,as a chronic,progressive disease,is one seriouscomplication of diabetes,which is characterized by the damage of nerves,blood vessels,skins,tendons,skeleton,and usually accompanied bygangrene in terminal ends of limbs.It is also regarded as a major reason ofnon-traumatic amputation.According to the classification of TraditionalChinese Medicine,diabetic foot is a kind of Tuo-gangrene.Due to theincreasing incidence of the disease,greater attention has been paid to theresearch of the disease by the domestic specialists and experts.Based on the new mechanism put forward by Professor Li,the author'tutor,"Malnutrition of the five Zang-organs;interior coagulation of dampheat;consumption of Qi and body fluid;thickness of blood and fluid;obstruction of phlegm",as well as the attention to the characteristics of thedisease,whose feature is deficient origin and excessive superficiality,considering the clinical expression of deficiency of Qi and Yin,togetherwith obstruction of stasis and accumulation of toxin,this research focusesmainly on the treatment of diabetic foot.As a result of the study,thetreatrnent principles—supplementing Qi and nourishing Yin;relievinginternal heat has been put forward by the author.In addition,TangzukangCompound capsule,as an effective prescription has been found and put intouse.Ideal Treatment effect has been achieved,which may guarantee thewidening of treatment theory of diabetes.Objectives:The purpose of the experiment is to find out the effect ofTangzukang Compound capsule on the contents and expressions ofinflammation factors in model rabbits with diabetic foot.A detaileddiscussion has been conducted concerning the mechanism of diabetic foot,which may lead to the exploration of the treatment mechanism of diabeticfoot by Traditional Chinese Medicine.Methods and results:Successfully establish the model of diabetic footin rabbits according to the literature materials.The methods of biochemistry,light microscope and immunohistochemistry were applied in the experiments.Based on the analysis of the effect of Tangzukang Compound capsule on the inflammation factor level—CR-P,IL-6,MMP-9,and TGF-β1,MMP-9,TβR-Ⅰexpression in diabetic foot model rabbits,it has been proved thatTangzukang Compound capsule may obviously reduce the levels ofinflammation factors,adjust TGF-β1,MMP-9 expression,and inhibit thecomposing of ECM in diabetic foot model rabbits.Tangzukang Compoundcapsule plays a role of repairing on the endomembrane of femoral artery indiabetic foot model rabbits.Conclusions:DF,characterized by deficient origin and excessivesuperficiality,and deficiency of Qi and Yin,can be treated effectively by theapplication of supplementing Qi and nourishing Yin;relieving internal heat.This research supplies practical methods to DF treatment,which representsthe holistic treatment and the thought of Traditional Chinese Medicine.Italso widens the horizon of treatment for diabetes from the perspective ofTraditional Chinese Medicine.After this treatment,the levels ofCR-P,IL-6and MMP-9 in the serum of model rabbits all have been improved,andperformed better compared with the cotroll group(p＜0.05).Adjustment ofthe expression ofTGF-β1,MMP-9 and inhibition of the composing of ECMin diabetic foot model rabbits also displays the fact that TangzukangCompound capsule plays the role of repairing on the endomembrane offemoral artery in diabetic foot model rabbits,and performed better comparedwith the cotroll group(p＜0.05).Inflammation factors and the appearance aswell as development of diabetes are relevant clearly.Therefore,attachmentshould be added in the clinical treatment of diabetes to the reinforcement ofanti-inflammation factors.The analysis above shows that Tangzukang Compound capsule mayeffectively inhibit and postpone the development of the disease;hence it canplay an active role in the treatment of diabetes.