| objectBased on the theory of Chinese medicine and literature records,the complicated factors used to build up chronic fatigue syndrome(CFS) rat model.In theory,study deeply of CFS pathogenesis oftraditional Chinese medicine to explain the relationship between the Qi collatetal channel and the CFS;through experimental research,test and verify the assumption that the Qi collateral channel related with the CFS,and explore the mechanism which reinforcing Qi and smoothing collateral channel improve the CFS.Materials and methodssort out systemly the relevant literature of the CFS,according to the theory of tradional Chinese medicine,tiding up the relationship of CFS with organs,the yin and yang,qi and blood,in-depth study of its causes,disease place,disease nature,and put forward boldly "CFS associated with the Qi collateral channel" hypothesis;according to the Pathogenesis nature of the Qi collateral channel for weakness,stagnation,by clinical practice,we pick out drug components of the prescription of reinforcing Qi and smoothing the collateral channel.Through the way of animal experiments,we observe the effect that prescription of reinforcing Qi and smoothing the collateral channel treat CFS,we can verify that the hypothesis is Sciencial and rational. Clean level 50 male Wistar rats were randomly divided into normal groups (10),model group(40).the modelgroup is adopted complicated factors to build up model.Modeling process:①movement build up model:make a rat-box of 160cmx80cmx60cm,the water depth of 30cm,the water temperature of about 4℃.40 rats are checked out daily,and swim in the cold water(4℃),30min each time for 21 days②sexy make model:modelrats mated with rut female rats each night,for 21 days.③stimulate make model:we nip one rat tail by hemostat which tip is enwraped by gauze,making the rat fight with each other.The living environment simulating human being's is bad,competition is fierce,not breaking skin and not bleeding as a limit,at the end of model,The number of each niped tail rat is basically the same,for 21 days,for 2 hours every time.Making model is over,we compared with the body weight,hair color,drinking water,food intake,activity and mental state such as the the differences of general performance in the determination of physical fatigue status of the normal group and rats model.And then divided model animal into the model group(B),the reinforcing Qi and smoothing collateral channel group(C) and the reingforcing Qi Group(D) and the smoothing collateral channel group(E),10 each group.Altogether with the normal group,the total number reach 50.After re-grouping,A group and B group are filled 2ml 0.9%sod.chloride to the stomach,C group,D group and E group are respectively filled with reinforcing Qi and smoothing collateral channel prescription,reinforcing Qiprescription,and dredging collateral channel prescription,for 14 days.The Reinforcing Qi and smoothing collateral channel prescription is consist of ginseng,astragalus,Aurantii,such as lung,The Reinforcing Qi prescription is consist of ginseng,astragalus. The smoothing collateral channel is consist of Aurantii and Long, according animal body surface area conversion,The equivalent rat dosages of person are converted,rats equivalent dosage are every kilogram of 50g.The weight ofcold water is 5 times than the drugs,and is immersed for 20 minutes.after the water is boiled,we use slow fire for 40 minutes.The liquid mixtures will be condensed to every milliter of 2g crude drug.3 kinds of drugs are saved in refrigerators at 0-4℃,and each oral 2ml.After the delivery,testing the content of the hypothalamus monoamine neurotransmitter,liver glycogen(LG),muscle glycogen,MDA,IL-1,β-EP, the levels of serum SOD activity,and other indicators of animal model to explore the relevant indicators of the animal model,using RT-PCR detected the expression of CRHmRNA,and such as indicators to observe the vitality of hypothalamus CRHmRNA expression bythe reinforcing Qi and smoothing collacteral channel treating the CFS model rat.Results of the above tested clearly indicate that reinforcing Qi and smoothing collacter channel have a effect on CFS rat model,and the micro-mechanism of reinforcing Qi and smoothing collacter channel treating CFS animal model.The resultsAfter the making model is over,the weight of model group animals reduce,the amount of water and food intake reducing significantly(with the normal group,p＜0.05),tail suspension test,fxed time is increased in 6 minutes,(compared with the normal group,p＜0.01).Open-field experiments, The time of the rats cliping hair a significant reduction in 6 minutes,in the number of moving through cells(P＜0.05),the rational gross time is very higher significantly(P＜0.05);exhaustive swimming test,the rat model of strength Dried up significantly and the time of swimming lower in 6 minutes (P＜0.01),The test results of Hypothalamus monoamine neurotransmitter content: animal model of hypothalamic DA group were significantly lower(p＜0.01),5-HT content was significantly higher(p＜0.01);after treatment DA content of reinforcing Qi and smoothing collacteral channel Group, reinforcing Qi Group,the smoothing collacteral channel than the model group,andhave significantly higher(p＜0.01),but the three group was no significant difference(p＞0.05).5-HT content is significantly low -er(p＜0.01)than the model group,three groups have clear effects,but compared with the smoothing the collacteral channel prescription,the reinforceing Qi and smoothing collacteral channel prescription was no sig -nifycant difference (p＞0.05),The effect of the reinforcing Qi and smo -othing collacteral channel prescription and the smoothing the coll act -eral channel prescription is better than reinforcing Qi Group(p＜0.05).The content of Liver glycogen test:normal group was significantly lower than the model group(p＜0.01),The treatment group was higher than the model group(p＜0.05),but there are no significant difference among the three groups (p＞0.05),the reinforcing Qi and smoothing collacteral channel prescription is higher than the reinforcing Qi Group(p＜0.05);that show that the effect of the reinforcing Qi and smoothing collacteral channel prescription is better than the reinforcing Qi Group;in the muscle glycogen content test:the normal model group is higher(p＜0.01),After treatment,the treatment muscle glycogen content is higher than the model group(p＜0.01),but there are no significant difference among the three groups(P＞0.05).SOD test results:The SOD content of animal model group is significantly lower than other groups,there are no significant difference in raising SOD activity among the reinforcing Qi and smoothing collacteral channel prescription,the reinforcing Qi prescription,andthe smoothing collacteral channel prescription(P＞0.05). MDA test results:the MDA content of model group was significantly higher than the normal group,that show that the damage ofthe model group animals, organs from free-oxygen radical is the Very prominent.Compared with the model group,the MDA content of the reinforcing Qi and smoothing the collacteral channel group are significantly lower(P＜0.05),that show that the reinforcing Qi and smoothing collacteral channel prescription have the role of preventing free-oxygen radical toxicity,in MDA content,there are no significant difference(P＞0.05)between the reinforcing Qi act and smoothing collacteral channel act,that makes it clear that the two groups can not block the free radical toxicity.Pituitaryβ-endorphin(β-EP) content detection: pituitaryβ-EP content of the model group of animals is significantly higher than the normal group(P＜0.01),which show that the means of complex factors making model have seriously effect on the metabolism ofβ-EP, Synthesis of its increase or the degradation slow down.The number of positive cells of the reinforcing Qi and smoothing collacteral channel group,the reinforcing Qi group,and the smoothing collacteral channel group are significantly lower than the model group(P＜0.05),close to the normal group (P＞0.05),the three groups could promptly reduceβ-EP in the pituitary,and have a role in lighting the chronic fatigue syndrome;the number of positive cells and the gray value of the reinforcing Qi and smoothing collacteral channel group compare with the reinforcing Qi Group and the smoothing collacteral channel group,there are significant different(P＜0.05),the reinforcing Qi and smoothing collacteral channel group proved more effective than the reinforcing Qi Group and the smoothing collacteral channel group.The IL-1 content of peripheral blood test:the content of peripheral blood IL-1 of animal model is significantly higher than the normal group(P＜0.01),which indicate the means of complex factors making model have seriously effect on the metabolism of IL-1,and its can increase the synthesis of IL-1 or Slow down degradation of it.After treatment,the three group of peripheral IL-1 level was lower than the model group(P＜0.01),the three groups can inhibit the activity of IL-1 and increase its degradation.The IL-1 content of the reinforcing Qi and smooth collacteral channel group compare with the reinforcing Qi group and the smooth collacteral channel group,there are significant difference(P＜0.05),which show the effects of the reinforcing Qi and smooth collacteral channel group than the reinforcing Qi group and the smooth collacteral channel group.Hypothalamus CRHmRNA expression detection,the CRHmRNA expression of the normal group is lowest,the result of the semi-quantitative test of it is significant lower than the model group(P＜0.01).the complex factors can reduce the CRHmRNA expression of animal model,which may be one of the reasons for the decline ofthe ability of model animal movement.after the treatment,the CRHmRNA expression of the treatment group is significantly lower than the model group(P＜0.01),which show the three groups can reduce the expression of CRHmRNA,which may be decrease the CRHmRNA expression of Hypothalamus of animals,and inhibit the function of the HPA axis,so as to lighten the fatigue of CFS,irritability,insomnia and so on.In the relationship of effect,the results of the CRHmRNA expression of the reinforcing Qi and smooth collacteral channel group compare with the reinforcing Qi group and the smooth collacteral channel group,there are significant difference(P＜0.05),the effects of the reinforcing Qi and smooth collacteral channel group than the reinforcing Qi group and the smooth collacteral channel group.And in the light of other experimental results,we know that the mechanism of the effect of the three group are different,the effect of the reinforcing Qi and smooth collacteral channel group is the best, and the effect of the reinforcing Qi group and the smooth collacteral channel group are nearly uniform.ConclusionBy complex factors,we manufacture rat model of CFS,and considere the causes and manifestations of the CFS.From the model of the animal after eating,drinking water,after making model,we observe the degree of fatigue,water intake,food intake,such as performances,the model's performance is uniform to CFS,which shows that the methods has a certain rationality.After the treatment of the reinforcing Qi and smooth collacteral channel prescription,the external symptoms and micro-mechanism of animal model have improved,which show that the reinforcing Qi and smooth collacteral channel prescription can prevent and treat CFS,and have a complicated mechanisms,It can regulate the neuroendocrine and immune network.Based on the above experimental results,we can conclude that the making modelsuch as swimming,labor room and stimulation can be used to copy the CFS model;CFS relate with Qi collacteral channel;the reinforcing Qi and smooth collacteral channel prescription can improve the clinical symptoms of CFS;the treatment mean from Qi collacteral channel theory has an the value of in-depth study,and has the foundation for future research of CFS.At the same time,there is great guiding value in in-depth study of the nature of the Qi collacteral channel.
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