Study About Model Combining Syndrome With Disease Of Breast Precancerous Change With Stagnation Syndrome Of The Liver In Rats

Objective:according to investigate the animal pattern of the depression syndrome ofthe liver breast precancerous,records breast precancerous change from the view ofangiogenesis,the hormone levels,in order to find a reasonable time to induction breastprecancerous in rats,and to provide a objective criterion for about breast precancerousmodel combining syndrome with disease in rats.Methods:combining the TraditionalChinese Medicine theory to experience of establish models,animal pattern of breastprecancerous was made by DMBA gavage,animal pattern of HMG was made by estroneand progesterone injection.stagnation syndrome of the liver modle was made by Jire law.Rats were randomly divided into 5 groups:the control group,the DMBA group,theestrone group,the DMBA folder tail group,the estrone folder tail group,these groupswere treated with different drugs.after 8 and 12 weeks,observing the shape of breast,microcirculation,5-HT,pathological changes of breast tissue,and the expression ofVEGF,Ki67,CD34,P63,C-erbB-2 by immunohistochemistry.Results:1,in the generalconduct,the comparative group more anastomosis the depression of the liver type to thetwo groups of the animal pattern(P〈0.01),there was significant difference (P〈0.05) inthe microcirculation and 5-HT;2,In the organization and morphology,after 8 weeks,theDMBA group's rate of breast precancerous higher than the DMBA folder tail group,after12 weeks,the DMBA group and the DMBA folder tail group is same (P〉0.05) in the rateof breast precancerous and canner occurrence,but higher than the estrone group and theestrone folder tail group(P〈0.01) 3,in Immunohistochemistry,the expression of VEGF,Ki67,C-erbB-2,CD34,P63 is difference in the DMBA groupand the DMBA folder tail group(P〈0.01),the two group is same repreat(P〉0.05).Conclusion:It is an successful method to induce animal pattern of thedepression of the liver type breast precancerous by gavage method and jire law.performance of general conduct,microcirculation,neuroendocrine are anastomosis withclinical features of stagnation of the liver type.the methods is to impact proliferating cellnuclear antigen,VEGF,expression of oncogene,shorten time about establish all models ofdisease,improve success rate.in addition,the study proved the expression of CD34 andP63 can differentiate Benign and malignant breast lesions,and provided reliable indicatorfor evaluating whether the animal pattern of breast precancerous is successful.