Efficacy Evaluation Of Chang’an Mistura In Treatment Of A Disease-syndrome Integrated IBS Model With Stagnation Of Liver And Spleen Deficiency

Objective:To observe the efficacy evaluation of Chang’an mistura in treatment of a rat model with stagnation of liver and spleen deficiency, and to compare its curative effect.Method:Newborn male rats for the experiment to build a new animal model, and they can be divided into six groups which contains normal group(A),model group(B), Chang’anyihao group (C),Chang’anerhao group(D),Tongxieyao Formula group(E) and Dicetel group(F),15rats in each group.Choosing the method of mother and child separated combining chronic bondage stimulus to establish the model.Since binding period8days,every group would be given NS, NS, Chang’anyihao(5.67g/kg), Chang’anerhao(6g/kg),Tongxieyao Formula(3g/kg) and Dicetel(2.7mg/kg) respectively, and dosing volume would be10ml/kg,once a day, for14consecutive days.Pay attention to observe the general condition of rats and the weight changin the experiment process, then kill the rats,taking around10ml blood serum from the abdominal aorta for index detection, colon5cm of the intestine for organizing index detection. Finally select total weight, abdominal echo score,5-HT,D-xylose from serum,IgA,IgG,EC and MC indicators to observe.With the statistical software of SPSS20.0to analysis,when carring on the statistical description,normal group would be compared with model group, and model group compared with model group, each drug group should to have statistically significant differences in results.Result:(1)before the trial groups rats mental state is good, each experiment in module rats gradually look tired, mental status of the efficacy after the treatment group rats improved to different degrees;(2)in addition to the normal group, the rest of the group of rats appeared weight loss, the model group weight loss is most obvious, and the differences between the treatment group, the differences between the Chinese and western medicine treatment group, but no significant difference between three groups of Chinese medicine treatment group;(3)in addition to the normal group, the rest of the group every pain threshold of rats decreased, the model group reduced degree is most obvious, and there is significant difference between each treatment group, and3 groups there are significant differences between traditional Chinese medicine treatment group;(4)normal set of5-HT, D-xylose content is higher than the rest of the group, with significant difference between normal group and model group, with no significant difference between the traditional Chinese medicine treatment group;(5)normal set of IgA and IgG levels lower than the rest of the group, and has a significant difference between the model group, and Chang’anyihao group no significant difference between the drug groups;(6)the normal set of EC and MC content are lower than the rest of the group, with statistically significant differences between model group, with no statistically significant difference between the treatment group.Conclusion:1.Changanyihao has the advantage in improving the IBS rat model.2. Changanyihao’s mechanism mainly includes the following aspects:(1) increase the spontaneous behavior of rats, to improve the depression;(2) improve visceral pain threshold of rats, increase the serum content of5-HT, reduce the body visceral sensitivity;(3) improve the function of small intestine absorption;(4) reduce the content of serum IgA and IgG, reduce EC and MC content in intestinal tissue, regulate the body’s endocrine and immune function.