| Objective1.1To reviewe iterature to understand the chronic superficial gastritis(CSG) research progress of Chinese and Western Medicine.1.2To investigated the treatment effect, of Xiangsha Liujunzi Decoction of CSG and clear the therapeutic advantage. Alpha1.3Animal experiments as an extension of the clinical research, to further explore the mechanism of action of Xiangsha Six Gentlemen Decoction tr-eatment of spleen qi deficiency type CSG.1.4Literature parts:reviewed and analyzed literature to understand the chronic superficial gastritis(CSG) research progress of Chinese and Western Medicine.Method1.1Clinical study parts:patients with CSG owing to Spleen-Stomach Qi Deficiency were assigned to2groups:the therapy group were treat with Xiangsha Liujunzi decoction and control group were treat with Conventional western medicine. To observe the traditional Chinese medicine syndrome, clinical efficiency, gastroscopy, Hp infection, the inflammatory pathology and quality of life and compared.1.2Animal experiments:Establishing CSG model rats with spleen-Stomach Qi deficiency type as the objective, then establish the blank control group, the model group,the Xiangsha Liujunzi decoction high-dose group, the Xiangsha Liujunzi decoction dose group, the Xiangsha Liujunzi decoction low dose group and Conventional western medicine group were treated with different processing factors, and then to observe the general status of rats in each group, HE staining of gastric tissue pathology, gastrointestinal motor function in rats. To observe the exopression of SP, INOS and5-HT in the brain and stomach by immunohisto-chemistry. To observe the exopression of INOS RNA, and5-HT RNA. By fluorescent PCR.Result1.1The clinical research:1.1.1The TCM syndrome score comparison of14days after treatment:the treatment group before and after comparison:epigastric fullness, epigastric discomfort, epigastric pain, belching and acid reflux, poor appetite and indigestion, fatigue after eating were significantly improved (p<0.05or p <0.01);the control group before and after comparison:epigastric pain, heartburn symptoms significantly improved (p<0.05or p<0.01). Epigastric fullness symptoms was improve better in the treatment group (p<0.05).1.1.2The TCM syndrome score comparison of28days after treatment:lthe treatment group before and after comparison:epigastric fullness after eating, epigastric discomfort, epigastric pain, heartburn, belching and acid reflux, poor appetite and indigestion, loose stool, fatigue, weakness8symptoms have significantly improved (p<0.05or p<0.01); the control group before and after comparison:epigastric fullness, epigastric pain, fatigue, weakness three symptoms have significantly improved (p<0.05or p<0.01). After eating epigastric discomfort symptoms was improve better in the treatment group (p <0.05).1.1.3The TCM syndrome score comparison of56days after treatment:the treatment group before and after comparison:epigastric fullness, epigastric discomfort after eating, epigastric pain, heartburn, belching and acid reflux, food poor appetite, loose stool, fatigue, weakness, tongue quality,10symptoms have significantly improved (p<0.05or p<0.01); the control group before and after comparison:epigastric fullness, epigastric pain, burning effort three symptoms significantly improved (p<0.05or p<0.01). The epigastric fullness, epigastric discomfort symptoms after eating were improve better in the treatment group(p<0.05).1.1.4The TCM syndrome score comparison of84days after treatment:the treatment group before and after comparison:epigastric fullness after eating, epigastric discomfort, epigastric pain, heartburn, belching and acid reflux, food less poor appetite, loose stool, fatigue, weakness, tongue10symptoms have significantly improved (p<0.05or p<0.01); the control group before and after comparison:epigastric fullness, epigastric pain, heartburn three symptoms significantly improved (p<0.05or p<0.01). The epigastric fullness, epigastric discomfort symptoms after eating were improve better in the treatment group (p<0.05).1.1.5The efficacy comparison in two groups after treatment:1.1.5.1TCM symptoms of the therapeutic effect:the total efficiency to56and84days after the treatment group compared with the control group has better therapeutic effect (p<0.05).1.1.5.2The gastroscopic effect:treatment group and control group has a significant difference in mucosal recovery (p<0.05). Hp infection:treatment group before and after contrast has no significant difference (p>0.05). The control group itself before and after contrast has a significant difference (p<0.05). contrast between two groups After treatment, there has a significant difference (p<0.05).1.1.5.3The pathological inflammation therapeutic effect:compared with the treatment group in the degree of inflammation, the control group is better, there has statistically significant (p<0.05). Treatment group before and after comparison has no significant difference (p>0.05). The control group before and after comparison has a significant difference (p<0.05), And the degree of inflammatory activity after treatment has no significant difference (p>0.05). there has no significant difference in before and after comparison in both group (p>0.05).1.1.6The quality of life effect:integral comparison of the treatment group and control group in each dimension has no significant difference (P>0.05); treatment group itself before and after treatment comparison of each dimension improved significantly (P<0.05or P<0.01). The control group itself before and after treatment comparison has significantdifference in6dimension.1.1.7Efficacy of the regression analysis:It shows that there has relationship between efficacy and the different treatment, as well as Chinese medicine symptoms (P<0.05or P<0.01), there has no relationship between efficacy and the SF-36scale points, Hp infection, the degree of pathological inflammation, and activity analysis.1.1.8Observation safety in two groups of patient:two groups of patients during treatment were not found significant adverse drug reactions.2.1The animal experiments:2.1.1The body weight of rats:after modeling, there has significant difference between model ing-made modules and blank group (P<0.01). Giving drug intervention, compared with the model group rats, the rats in all dose group of Xiangsha Liujunzi decoction were significantly increased, there was significant difference (P<0.05). There has no significant difference among each dose group of Xiangsha Liujunzi decoction, compared with model group, Conventional western medicine group, has no significant increase in body weight after intervention.2.1.2Food quantity intake in rats:after modeling, there has significantly different between each modeling group and the blank group (P<0.01). Given the drug intervention, compared with the model group rats, Xiangsha Liujunzi decoction each dose group rats eating volume increased significantly, there are significant differences (P<0.01), while there has no significant difference among each dose group of Xiangsha Liujunzi decoction, but eating volume of these groups still lower than the blank group rats (P<0.01).2.1.3Water quantity intake of rats:there has significantly different between each modeling group and the blank group (P<0.01or P<0.05). Given the drug intervention, each dose group compared with the model group rats Xiangsha Six Junzi Decoction in rats drinking water volume increased significantly, there are significant differences (P<0.01or P<0.05), while Xiangsha liujunzi Decoction dose group between the most significant improvement of the high dose group, but Xiangsha each dose group was still lower than the blank water intake of rats (P<0.01).2.1.4Gastric emptying of rats:compared with the blank group rats, except Xiangsha high dose group, other groups gastric emptying is weaker (P<0.01or P<0.05). Compared with the model group, gastric emptying are better in Xiangsha each dose group.(P<0.01or P<0.05).2.1.5Ordinary light microscope pathology:Compared with blank group, other groups of gastric mucosa presents lymphocytic infiltration of chronic inflammatory changes of the local vascular little congestion, inflammatory cell infiltration in the mucosa shallow glands remain intact. Compared with model group, Xiangsha inflammation in each dose group must reduce the inflammation for Purell ketone group to reduce the most obvious.2.1.6In rat stomach and brain SP expression:blank group, in addition to Xiangsha outside the high dose group, â… group the stomach of SP expression (P<0.01or, P<0.05). Compared with model group rats, Xiangsha each dose group rat gastric SP expression were weaker (P<0.01). Compared with the model group were also weak (P<0.01), for the expression of SP Puri ketone group stomach. And compared with the control group, rats, other than the addition to Xiangsha high dose group, â… brain SP expression are stronger (P<0. Olor P<0.05), compared with the model rats, Xiangsha each dose group SP expression of rat brain was no significant difference (P>0.05). Compared with the model rats, no significant difference for the expression of SP Puri ketone group stomach.2.1.7In rat stomach and brain INOS expression:can be found, compared with the control group rats, in addition to low Xiangsha, other than in the high dose group, the expression of more than groups of stomach INOS2strong (P<0.01or P<0.05). Compared with model group rats, the expression of the Xiangsha high dose groups stomach INOS2weaker (P<0.01). Rats compared with the model group, on behalf of the stomach the INOS the expression of the the Puri ketone group, no significant difference (P>0.05); compared with the control group rats, in addition to the in Xiangsha in the high dose group, I group of brain INOS2expression of strong (P<0.05). Compared with model rats, Xiangsha each dose group rat brain INOS expression was no significant difference (P>0.05). Compared with the model rats, no significant difference for the stomach the INOS the expression of the the Puri ketone group.2.1.8In rat stomach and brain5-HT expression:blank rats In addition to the model group, the expression of I group of the stomach of5-HT compared with those without a significant difference (P>0.05). Compared with model rats, Xiangsha in weak expression of the rat stomach of the high dose group of5-HT (P<0.01). Compared with the model rats, for the expression of the Puri ketone group gastric5-HT was no significant difference (P>0.05); comparison with the control group rats, in addition to low Xiangsha, other than in the high dose group, more than all group of brain5-HT in the expression of a strong (P<0.05). Compared with model rats of Xiangsha each dose of5-HT in the brains of rats showed no significant difference (P>0.05). Compared with the model group were, for the expression of the Puri ketone group gastric5-HT was no significant difference (P>0.05).2.1.9Stomach, brain tissue5-HTR1A mRNA expression:blank rats, in addition to Xiangsha outside the high dose group, more than groups of stomach5-HTR1A mRNA expression were higher (P>0.05). Compared with model rats Xiangsha, the high dose group rat gastric-HTRIA mRNA expression was no significant difference (P>0.05). Compared with model rats, for of Puri ketone group stomach-HTRIA mRNA expression was no significant difference (P>0.05); compared with the control group rats, except Xiangsha low, medium and high dose group outside, I each group of brain5-HTRIA mRNA expression were higher (P<0.05or P<0.01). Model group rats compared with less Xiangsha high-dose group rat brain5-HTR1A mRNA expression (P<0.01). Compared with the model group were, for of Puri ketone group stomach-HTRIA mRNA expression was no significant difference (P>0.05).2.1.10Stomach, brain tissue of INOS mRNA in expression:blank group rats, only the stomach of INOS mRNA expression in model group a strong (P<0.05). Compared with the model group rats Xiangsha high dose group rat stomach of INOS mRNA expression less (P<0.01). Compared with model rats for Puri ketone groups stomach of INOS mRNA expression was no significant difference (P>0.05); compared with the control group rats, the same model group, the stomach of INOS mRNA expression (p<0.05). Compared with the model group rats Xiangsha high dose group of the rat brain of INOS mRNA expression less (P<0.05). Compared with the model rats, for Puri ketone group stomach of INOS mRNA expression was no significant difference (P>0.05).Conelusion1.1The clinical research component:1.1.1Xiangsha Liujunzi soup to improve the TCM symptoms, presents the following advantages:1.1.1.1rapid onset.1.1.1.2symptoms improve overall.1.1.1.3Dyspeptic symptoms improved better. Such as epigastric fullness, epigastric discomfort after eating symptom efficacy compared with the control group better.1.1.1.4The improved gastrointestinal symptoms outside the better.1.1.1.5long-term efficacy.1.1.2Xiangsha Liujunzi soup and Conventional western medicine poor treatment of Hp eradication.1.1.3Western medicine in the improvement of the degree of inflammation in patients with Conventional western medicine decoction group is superior to traditional Chinese medicine Xiangsha Liujunzi.1.1.4The efficacy of the quality of life showed the following characteristics:theâ‘ Xiangsha Liujunzi Tang Treatment of Spleen Deficiency CSG to improve on the quality of life in patients with more comprehensive. Theâ‘¡Xiangsha Six Junzi Decoction and Conventional western medicine in improving clinical symptoms, can effectively improve the quality of life of patients.1.1.5The efficacy of therapeutic drugs and TCM symptoms improve correlation with the SF-36scale points, Hp infection, the degree of pathological inflammation, and the activity degree wireless association.2.1The animal experiments:2.1.1Xiangsha Six Junzi Decoction can improve the weight loss of the spleen and stomach Qi CSG rats in the short term, but difficult to reach normal levels in the short term.2.1.2The Xiangsha of high doses of Six Junzi Decoction can improve gastric emptying function of the spleen and stomach Qi CSG, can reach normal levels.2.1.3Improvement of mucosal inflammation, Xiangsha Liujunzi soup no more prominent advantages.2.1.4spleen and stomach Qi CSG model, there may be increased SP content of the lesion; Xiangsha Liujunzi soup, high doses can reduce the stomach, brain expression of SP, and basically reached the normal level. In this regard, Conventional western medicine Xiangsha high dose group, especially the efficacy of high dose group there are some gaps.2.1.5Xiangsha in dose can be reduced to central nervous system and peripheral nerve INOS expression; peripheral gastric nervous system INOS expression of strong and weak may be the central nervous system of INOS RNA expression intensity associated; may the Xiangsha Liujunzi soup to improve the experimental rat gastric emptying function mechanism.2.1.6Xiangsha, dose can be effectively lowered the expression of the central nervous system and peripheral nerves of5-HT; gastric5-HT expression of the strength of central nervous system5-HT1A receptor RNA expression strength of the association; through the digestive tract and central nervous5-HT and5-HT1A effectively lowered, may be Xiangsha Liujunzi soup to improve the experimental rat stomach indigestion state mechanism.
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