| Objective:To observe the effects of XSLJZD ecoction(XSLJZ)on levels and mRNA of IL-6,IL-17 and protein expression of ERK1/2 of gastric mucosa in chronic atrophic gastritis(CAG)progress of rats with spleen-stomach deficiency and to analyze its regulation mechanism of XSLJZ on gastric mucosa of CAG model rats,and to study its molecular biological mechanism of preventing and treating CAG.In the end,to provide the theory foundation and pharmacodynamics foundation for XSLJZ preventing and treating CAG in the clinic.Methods:Rats were divided into 2 groups:normal group and model group through random number table.The model of CAG rat with spleen-stomach deficiency type was induced by synthetic methods,Which were divided into model group,XSLJZ high-,medium-,low-dose group,positive control group.10 mL/kg of distilled water was given to model and normal group every day;24,12,6g/(kg·d)of XSLJZ was separately given to XSLJZ high-,medium-,low-dose group;0.30g/(kg·d)of mycin was given to positive control group for gavage for 120 consecutive days.Using ELISA method to detect the expression levels of IL-6and IL-17;levels and mRNA of IL-6,IL-17 and ERK1/2 were detected by PCR and protein expression of ERK1/2 in gastric mucosa tissue were detected by western blot.Results:1 Macroscopic observation:sensitive reaction,normal diet,a good mental state,hair soft,thick and shiny and brown granular are in normal group rats.Half of the rat model of CAG began to appear low spirit,lethargic,dry coat and reduced to eat after sixth weeks;it would be worse after eighth weeks;After treatment,the symptoms began to ease,some disappeared,the fur was shiny and diet had a significant recovery than before.2 The changes of pathologic morphology of gastric mucosa:there were intact epithelium,glandular plump,neat arrange,clear cytoplasm,cell monolayer columnar glandular epithelium and gland clear demarcation in the normal group;however there were superficial epithelial erosion,glandular epithelial cell degeneration,less mucous layer,glands atrophy,basal gland epithelial hyperplasia,and cell infiltration in the model group;high-dose group had structure order and a little glandular organ thinner;the mucous membrane of medium-dose group wasmore complete,the glands were rich,the permutation was more orderly,the inflammatory cell infiltration was not obvious,and I could see the cup cell.The mucosa of low-dose group was slightly thinner,a small amount of gland and inflammatory cell expanded,atypical hyperplasia had a little developed.The mucosa of positive control group was thinner,the permutation was more orderly,atypical hyperplasia hadn’t developed.3 The changes of level of IL-6 and IL-17:Compared with the normal group,the level of IL-6 and IL-17 increased significantly in the model group(P<0.05),Compared with model group,the level of IL-6 and IL-17 showed significantly decreased in XSLJZ high and medium-dose group(P<0.05),the level of IL-6 and IL-17 was not obvious in low-dose and positive control group(P<0.05).4 The changes of transcriptional level of IL-6,IL-17 and ERK1/2 of gastric mucosa:Compared with normal group,the transcriptional level of IL-6 and IL-17 increased significantly(P<0.05)and the transcriptional level of decreased significantly in the model group(P<0.05);Compared with model group,generally living conditions of XSLJZ groups were improved significantly,mRNA and the level of IL-6 and IL-17 showed significantly decreased(P<0.05);the level of IL-6 and IL-17 was not obvious in low-dose and positive control group(P>0.05);the content of ERK1/2 showed significantly increased except the low-dose group(P<0.05).5 The changes of protein expression of ERK1/2 and P-ERK1/2 of gastric mucosa:Compared with normal group,the protein expression of ERK1/2 decreased significantly in model group(P<0.05),Compared with model group,the protein expression of ERK1/2increased in XSLJZ high and medium-dose and positive control group(P<0.05),the protein expression of ERK1/2 was not obvious in low-dose group(P>0.05);Compared with normal group,the protein expression of P-ERK1/2 was obviously increased in model group(P<0.05),Compared with model group,the protein expression of P-ERK1/2 was obviously decreased in XSLJZ high and medium-dose group(P<0.05),the protein expression of ERK1/2 was not obvious in low-dose and positive control group(P>0.05).Conclusion: XSLJZ can decrease the level of IL-6 and IL-17,arouse the protein expression of ERK1/2 and restrained the expression of P-ERK1/2,It was suggested that XSLJZ had protective effects on gastric tissues of CAG rats with spleen-stomach deficiency.Itwas possible that this was one of the possible mechanisms of XSLJZ for suppression the development of CAG inflammation,to provide the theory evidence for XSLJZ to prevent and cure CAG. |
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