Influence Of RKABF On Interrelated Gene Of Ovarian Local Cells In PCO Rat

Object:To explore the effects of Restoring Kidney and Activating Blood Formula (RKABF) has on the expression of interrelated gene in Ovarian local Cells of the rats suffering from rats model of polycystic ovary. So, the study is to expound the relevant function mechanism of RKABF from the aspect of PCOS.Methods:We select seventy-nine SD female rats of six-week old who should have the regular estrum of SPF standard. The rats which feeding in the environment that is consistent with SPF-class animal requirements and these random samples are divided into two groups for contrast:the normal group and the model group (the rate is 1:5), and then the PCO Model of the rats are set by hypodermically injecting Letrozole (LE), normal group are set by 1% Carboxymethyl(CMC). We total need 21 days to complete this step. After this the PCOS Model of the rats and the normal group randomly divided into five groups for contrast, i.e. the first group (model group), the second group(RKABF group of high dose), the third froup(RKABF group of average dose), the fourth group(RKABF group of low dose), the fifth group(western medicine group), and the nomal group,a total of six group, then effect them with different medicine:0.9%Nacl, the RKABF decoction, Ethinylestradiol and Cyproterone Acetate Tablets. This process is a continuous work 3 days once a day, the rest a day, for a total work of 21 times. After that, We randomly selected eight rats in each gorup, and we choose one side of the rats used to separed the ovarian granulose cells and check the expression of interrelated gene in Ovarian local Cells of the rats. Real-time quantitative PCR determination of gene expression values to explore the effect of RKBAF from the aspects of PCOS rats'ovarian local cells.Results:Firstly, Compared normal group with control groups without treatment, there are statistical significance in 13 gene; Secondly, The Expression of CYP17 has statistical significance between low dose group and normal dose group of BSHYF;Thirdly, There are more influence on granular cell the thecacells about the of gene expression;Forthly, There are statistical significance in gene AR,IGFR and FSHR on granular cell between the group with control and BSHYF groups.Conclusion:The BSHYF can influence the expression of gene of the ovary of PCO rats.Maybe it related to the change of gene receptor...