The Effect Of Jian Pi Gu Yi Decoction On The Inflammatory Agents In Serum And TNF-α,IL-6 MRNA Expression In Hepatic Tissue Of Diabetes Mellitus 2 Rats

Objective:To investigate the effect of Chinese medicine Jian Pi Gu Yi decoction on the concentration of TNF-α, IL-6 and CRP and the gene expression of TNF-αand IL-6 in liver of rats with diabetes mellitus 2, and discuss the role of TNF-a and IL-6 in the cause of the disease and the therapeutic action of the traditional chinese medicine.Method:Rats model of Type 2 diabetes mellitus(T2DM) were induced with intraperitoneal injection of STZ and high fatty feeding. Wistar rats were divided into five groups:normal control, T2DM model group, Jian Pi Gu Yi decoction 1 group, Jian Pi Gu Yi decoction 2 group and Rosiglitazone group. The rats in experimental groups were fed with standard dose of Jian Pi Gu Yi decoction, high dose of Jian Pi Gu Yi decoction and Rosiglitazone respectively. Fasting blood glucose and insulin, cholesterol. triglyceride, TNF-α, IL-6 and CRP were tested in all the rats after 4 weeks of treatment. The mRNA expression of TNF-αand IL-6 were tested with RT-PCR and the histio-strcture of liver were observed.Results:(1) Compared with normal control group, fasting blood glucose in other groups was higher (p<0.01). And compared with T2DM model group, fasting blood glucose was decreased significantly in Jian Pi Gu Yi decoction 1 group, Jian Pi Gu Yi decoction 2 group and Rosiglitazone group(p<0.01). However, there was no significant difference in Jian Pi Gu Yi decoction 1 group, Jian Pi Gu Yi decoction 2 group and Rosiglitazone group(P>0.05). (2) Compared with normal control group, fasting blood insulin in other groups was higher (p<0.01). However, there was no significant difference in T2DM model group, Jian Pi Gu Yi decoction 1 group, Jian Pi Gu Yi decoction 2 group and Rosiglitazone group(P>0.05). (3) Compared with normal control group, serum cholesterol in other groups was higher (p<0.01). And serum cholesterol in Rosiglitazone group was decreased significantly compared with T2DM model group and Jian Pi Gu Yi decoction 1 group (p<0.01). However, there was no significant difference between Jian Pi Gu Yi decoction 2 group and Rosiglitazone group(P>0.05).(4) Compared with T2DM model group, serum triglyceride in other groups was lower (p<0.01). And compared with Jian Pi Gu Yi decoction 2 group, serum triglyceride Jian Pi Gu Yi decoction 1 group was increased significantly(p<0.01). However, there was no significant difference in Jian Pi Gu Yi decoction 1 group. Jian Pi Gu Yi decoction 2 group and Rosiglitazone group(P>0.05). (5) Compared with normal control group, serum HDL in other groups was lower (p<0.01). There was no significant difference between Jian Pi Gu Yi decoction 1 group and Rosiglitazone group. And the level of serum HDL in Jian Pi Gu Yi decoction 2 group was higher than Jian Pi Gu Yi decoction 1 group(p<0.01) and Rosiglitazone group(p<0.05). (6) Compared with normal control group, serum LDL-C in other groups was higher (p<0.01). And the level of LDL-C in Rosiglitazone group was lower than Jian Pi Gu Yi decoction 1 group and Jian Pi Gu Yi decoction 2 group (P<0.05). However, there was no significant difference between Jian Pi Gu Yi decoction 1 group and Jian Pi Gu Yi decoction 2 group. (7) Compared with normal control group, serum TNF-a in other groups was higher (p<0.01). And the level of serum TNF-αin Jian Pi Gu Yi decoction 2 group was lower than Jian Pi Gu Yi decoction 1 group and T2DM model group(p<0.01). However, there was no significant difference between Jian Pi Gu Yi decoction 2 group and Rosiglitazone group(p<0.05).(8) Compared with normal control group, serum IL-6 in other groups was higher (p<0.01). However, there was no significant difference in Jian Pi Gu Yi decoction 1 group, Jian Pi Gu Yi decoction 2 group and Rosiglitazone group(p<0.05). (9) Compared with normal control group, serum CRP in T2DM model group, Jian Pi Gu Yi decoction 1 group and Rosiglitazone group were higher (p<0.01), and there was no significant difference between normal control group and Jian Pi Gu Yi decoction 2 group(p>0.05).And the level of CRP in Jian Pi Gu Yi decoction 2 group was lower than Jian Pi Gu Yi decoction 1 group (p<0.05). There was no significant difference in Jian Pi Gu Yi decoction 1 group, Jian Pi Gu Yi decoction 2 group and Rosiglitazone group(p >0.05).(10) When observing the HE staining of liver, the histio-strcture in normal control group was normal. And the steatosis and hepatic inflammatory infiltration around the small tube in Jian Pi Gu Yi decoction 2 group were reduced compared with Jian Pi Gu Yi decoction 1 group, Rosiglitazone group and T2DM model group. Compared with T2DM model group. the steatosis and hepatic inflammatory infiltration around the small tube were reduced in Jian Pi Gu Yi decoction 1 and Rosiglitazone group. (11) Compared with T2DM model group, the mRNA expression of TNF-αin Jian Pi Gu Yi decoction 1 group. Jian Pi Gu Yi decoction 2 group and Rosiglitazone group were decreased(p<0.01) but was higher than normal control group(p<0.01). There was no significant difference in Jian Pi Gu Yi decoction 1 group. Jian Pi Gu Yi decoction 2 group and Rosiglitazone group(p >0.05). (12) Compared with normal control group, the mRNA expression of IL-6 in other groups was higher (p<0.01). and compared with T2DM model group, the mRNA expression of IL-6 in Jian Pi Gu Yi decoction 1 group, Jian Pi Gu Yi decoction 2 group and Rosiglitazone group were decreased(p<0.01). The mRNA expression of IL-6 in Jian Pi Gu Yi decoction 2 group was significantly decreased than Jian Pi Gu Yi decoction 1 and Rosiglitazone group(p<0.05).Conclusions:Jian Pi Gu Yi decoction can decrease Fasting blood glucose and cholesterol in diabetes mellitus 2 rats, Jian Pi Gu Yi decoction can decrease serum TNF-a and IL-6,too.and the effects may be the results of its decreasing action on the expression of TNF-αand IL-6 in rat liver.