| Objective:The mechanism of Ci Wu Jia on improving sleep in drosophila was studied using the microarray of whole drosophila genome. Methods:In this study, the effects of Ci Wu Jia on improving sleep of normal flies and flies sleep-derprivation models was determined by using the Drosophila activity monitoring technology. The flies models of sleep-deprivation was maked by the alternative light-dark stimulating at night. On this basis, the acting mechanism was explored by using the microarray technology of whole drosophila and bioinformatics data analysis techniquesResults:1. The climbing index of female flies aged 1-day-old to 32-day-old maintained stability at the highest level; the climbing index of male flies aged 1-day-old to 22-day-old remain stability at the highest level; the climbing index female and male flies aged 7-day-old to 17-day-old were no significant difference.2. The sleeping time and sleep consolidation index of female flies was increasing with age from 2-day to 12-day, and decreasing with age from 12-day to 32-day; the sleeping time and sleep consolidation index of male flies was increasing with age from 2 day to 22 day.3. The sleeping time of female fruit flies total sleeping time was significantly less than the male fruit flies(p<0.01); female flies sleep mainly at night, and the sleeping time of male flies during the day and night was no significant difference.4,The alternative light-dark stimulation can reduce sleeping time and sleeping index of female and male flies significantly(p<0.01).5. The sleeping time and sleeping consolidation of sleep-deprivation models of female and male flies was recoveried by administering diazepam at the concentration of 0.125 mg/ml (P <0.05).6. CWJ can increase sleeping time of the male and female flies significantly (p<0.05) at the concentration of 0.125% to 1% , and the sleeping consolidation index was increased (p<0.05); CWJ can increase the sleeping time(p<0.01) and sleeping consolidation index(p<0.01) significantly at the concentration of 0.25%.7. CWJ of 0.25% can significantly increase the sleeping time (p<0.01) and sleeping index (p<0.01) of female flies administration 4d-7d; CWJ of 0.25% can significantly increase the sleeping time (p<0.01) and sleeping index (p<0.05) of male flies administration 4d-7d.8. CWJ of 0.5% and 1% can increase sleeping time of the sleep deprivation model of female flies(p<0.05), CWJ can increase sleeping index of the sleep deprivation model of female flies(P <0.05) significantly at the concentration of 0.25%-1%; the sleepin time and sleep index of the male flies sleep deprivation model were not significantly affected by CWJ.9. Administration of 2 to 7d of CWJ of 1% can significantly increase the female flies sleep deprivation sleep time (p<0.01), administration of 3d-7d can significantly increase the female flies sleep index (p<0.05)..10. CWJ(concentration of 1%, administration 4 days) can changed expression of 194 genes in the head of fly, 80 genes up-regulated, and 114 genes were down-regulated; night light-dark stimulus can changed expression of 184 genes in the head of fly, 83 genes up-regulated, and 101 genes were down-regulated; the interaction of CWJ(concentration of 1%, administration 4 days) and night light-dark stimulating can changed expression of 685 genes in the head of fly, 188 genes up-regulated, and 497 genes were down-regulated.Conclusion:1. The climbing index of flies decreased with increasing age.2. The sleeping time and sleeping index of female flies changes with age, before age 12 tended to increase, then decreasing; the sleeping time and sleeping index of male flies changes with age, 2day age to 22 days of age tended to increase.3. CWJ can improve male and female fruit flies sleep-wake cycle at the best dosage regimen: CWJ of 0.25%, delivery time≥4days.4. CWJ can improve sleep-wake cycle of sleep deprivation model of female fruit flies at the best dosage regimen: CWJ of 1%, delivery time≥3days; CWJ was no effect on sleep-wake cycle of male flies sleep deprivation model.5. CWJ improve the role of fruit flies sleep, is dco, slo and DopR2 major players such as genes involved in the process of multi-gene, multi-gene co-regulation results.
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