The Study On The Effects Of Semen Ziziphi Spinosae Freeze-Dried Powder On Improving Sleep Using Drosophila Melanogaster As The Model Organism

As a basic behavior and an important physiological phenomenon in human,sleep has an important relationship with social behavior,dietary behavior and sexuality.Normal person has a sleep-wake cycle per 24/25h in nature.Sleep quality has an important influence on people's lives and health. Scientists have confirmed that not only sleep can maintain the vitality of the body,but also improve the capacity of information storage and processing by dreaming.For this reason,sleep studies are concerned more and more by people.However,along with the rapid rhythm of modern social living,the exaggeration of the work pressure.Sleep disorder has been a common disease and some other diseases' reason.The adjusting process of sleep is very complicated.Since sleep problems appeared,People have been making efforts to the study on the mechanism all the time.Recently,the establishment of models for study of human disease using drosophila melanogaster has been becoming the new research luminescent spot.Many features of sleep in drosophila are similar to human sleep,such as behavioral,pharmacological,molecular,and genetic approaches.So,drosophila with well-established genetics had become an ideal model organism for sleep research.In our studies,we knew the change of activities in drosophila by testing their climbing ability.Then we used an Infrared Drosophila Activity Monitor System(DAMS;Trikinetics Inc.USA) to record sleep state of drosophila.We observed and analyzed the recording data for continuous 24 hr,and defined sleep time that drosophila's quiescing time is exceed 5 min.We investigated how can sex and age affect total sleep time and sleep-wake cycle in drosophila with index of sleep time by analysis of data.Then we studied the dose-effect and time-effect of Semen Ziziphi Spinosae Freeze-dried Powder in drosophila sleep improvement by selecting the 7-day-old drosophila as observation of objects.At last,we verified the effect of Chinese medicine Semen Ziziphi Spinosae freeze-dried powder on sleep improvements in sleep-deprived Drosophila by two different sleep deprivation ways.First,The activities of drosophila maintaining the highest level steadily in 7-17 days.Second,The activity of fruit fly presents certain rhythmicity in 24h.Third,Our results indicated:The young fruit flies have a long sleep time and a good continuity.The sleep was fragmented gradually with the growth, and have similar features with human sleep.Fourth,there are some difference between male and female in sleep. Female flies sleep mainly at night,but male sleep mainly at noon and night. Male flies have a longer sleep time than female.Fifth,2%drug substratum for dose,3 days for delivery time is best to male(p＜0.01);4%o drug substratum for dose,7 days for delivery time is best to female(p＜0.01).Sixth,Semen Ziziphi Spinosae freeze-dried powder has a effect on sleep-deprived flies induced by light.Last,Semen Ziziphi Spinosae freeze-dried powder has a effect on sleep-deprived flies induced by mechanical stimulation.