Development And Analysis Of Mouse Lung Cancer Model Induced By Passive Smoking And Antioxidant Intervention

Object: Worldwide, the lung cancer is not only the most common tumor, but also the tumor of supreme incidence and death rate in all malignant tumor. Smoking is the main cause of lung cancer. So, explore the reason of lung cancer and ask the clear protective measure effective to passive smoker to seem very important and urgent. In this study, we will use mouse lung cancer model induced by passive smoking to explore the reason of lung cancer and mechanism of intervention in oxidant and anti-oxidant, immune response, apoptosis and gene level. In the end, we will give useful method and theoretical foundation to prevent the lung cancer induced by passive smoking.Method: 1 Development of mouse lung cancer model induced by passive smoking,BP combines 17β-estradiol model, antioxidant intervention model and Embryo lung cells translation model induced by cigarette smoke extract(CSE).2 To measure SOD, MDA , GSH-PX , ROS and VE level of serum and mitochondria;ELC DNA content, apoptosis and cell cycle of mouse lung cell and ELC; the spleen Lymphocyte transformation index; the length of telomere of blood and lung DNA; the mice lung gene change using Affymetrix MOE 430 2.0 gene expression chip.Results and Conclusion:1.It is succeeds in using Kunming strain mice to produce lung tumor model and antioxidant to intervene model by inhalation of tobacco smoke that have established the good foundation for the research of next lung cancer mechanism. There are gender differences in the incidence and multiplicity of lung cancer,that of female mice in three vitamin E(VE) intervene group are all higher than that of male mice. 2.The incidence of BP combines E2 group is obvious higher than BP simple function group,male intervention group and three control group,that prove the estrogen has facilitation to the development of female mouse lung cancer. Lung cancer incidence of every VE intervene group is higher than every control group and BP group alone obviously. The high incidence of lung cancer of VE intervene group proves VE facilitate to female mouse lung cancer indirectly,...