Development And Analysis Of Mouse Lung Cancer Model Induced By Estrogen Combines BP

Object: Worldwide, the lung cancer is not only the most common tumor, but also the tumor of supreme incidence and death rate in all malignant tumor.Estrogen signal may play very a important part in the development of lung cancer in female who smoke. It is very important and urgent to explore the reason and mechanisms of lung cancer in female who smoke. In this study, we have developed the mouse lung cancer model induced by BP combines 17β-estradiol, explored the founction of estrogen in formation of lung cancer induced by BP, and the mechanisms of estrogen promoting BP carcinogenesis in female. This will provide useful methods and theoretical foundation in prevention the lung cancer of women who smoke.Method:1.Development of mouse lung cancer model induced by BP combines 17β-estradiol model.2.Measure SOD,MDA,GSH-PX,ROS and VE level of serum;in order to determine the oxygen damage of lung tissue.3. Immunohistochemical staining was used to detect the expression of ERαand ERβin lung.4. The real-time RT-PCR was performed to measure the expression of ERβand NF-kB mRNA. Results and Conclusion:1. We have developed the mouse lung tumor model by BP combines 17β-estradiol model using Kunming strain mice. The lung cancer incidence of BP combines E2 group is obvious higher than BP group, male intervention group and three control group. This is revealed that estrogen can promote the carcinogenisis of B[a]P in female mouse. Lung cancer incidence of every VE intervene group is higher than every control group and BP group alone obviously. This results maybe indicate that VE also enhance BP carcinogenesis in female mouse indirectly,2. The higher the incidence of lung cancer in the groups, the more the ROS concentration. It is showed that BP combined E2 causes mouse lung cancer also through oxidizing damage pathway.3. ERβprotein is expressed in different lung cancer and the highest expressing rate can reach 83%, but there is very low expressing rate in normal lung tissue. ERαis expressed in lung cancer and normal lung tissue. The highest expressing rate can reach 100%, the lowest is 0%. However, There is no relationship the expression of ERαand tumor occurrence.4. ERβmRNA is expressed in lung cancer, and it is accordant with expression of protein level. These indicate that ERβmaybe have close relation to lung cancer in female who smoke. It also illustrates that estrogen play a role in lung cancer through ERβ.5. There are no principal expression of NF-kB mRNA in different groups.