Impact Of Passive Smoking On A Part Of Tissue Morphologically Of Mice

Harm of tobacco has become one of the most serious public problems in the world, which does harm to people's health. At present, there are 1.1 billion smokers worldwide. Nearly 5 million cases of avoidable death occur due to smoking each year. There are currently about 350 million smokers in China, ranking top of the world. One million people die from the diseases related to smoking every year. A large number of clinical observation and animal experiments prove that smoking can damage the body's various tissues and organs, causing cancer, hypertension, coronary disease, apoplexy, peptic ulcer, chronic bronchitis, emphysema and other diseases. It is the fact that passive smoking do more harm than active smoking does. Most research about passive smoking is focusing on the study of the epidemiology clinically, and rarely on the morphological impact on body tissue.In this study, as the material, the 4-week-old male Kunming mice (weight 23.1±3.0g / only), a total of 60, were divided into three groups randomly: A group (control group), B group ( 1 cigarette / times×5 times / day) and C (2 cigarettes / times×5 times / day), 20 in each group. The process of exposure to toxicant carried out in the exposed-cylinder by cigarette tobacco SHANCHA from Yun Group Co., Ltd. The 60 were treated in passive smoking per day form 8:30 am to 4:30 pm for sustained exposure 4 weeks. First, using the method of rat orbital venous plexus blood sampling, then made the mice dead with cervical vertebra quick disconnect, take bilateral testis, kidney, lung, spleen and part of jejunum, and then make tissue sections of these organs with paraffin method , H-E staining, using the micro-processing of digital image analysis system to observe the changes in organizational structure; the hemoglobin content of blood samples was determination by Salle's colorimetry. These indicators to study and explore impact of passive smoking on the part of tissue morphologically of mice, furthermore explore impact of its on the morphological tissue of human, and then provide a theoretical and scientific basis for make comprehensive study of impact of passive smoking on human health.The results indicated that:1.The experimental group's day-drinking and day-eating was significantly (P <0.05) less than the control group, from two weeks after exposure, the average weight of the experimental group significantly (P <0.05) lower than the average weight of the control group, but between the two experimental groups there is no significant difference (P <0.05), hemoglobin content in the experimental group (13.41±0.34 and 13.37±0.33) is significantly less than the control group (14.56±0.30). (P <0.05)2. The experimental group's bronchiole and the terminal bronchiole epithelial cells of the ciliary get loss, cell shrinkage get loss or even fall off; some alveolar tubes, alveolar sac, alveolar show signs of damage, and even accompanied by hyperemia, marked hyperplasia of alveolar septa, accompanied by infiltration of inflammatory cells, the extent of the damage Group C is more serious than Group B. 3. Experimental group's glomerular hyaline get degeneration, fibrosis, some tubular (proximal tubule, distal convoluted tubule) become atrophy, the lumen got significantly narrowed, the interstitial connective tissue occurred hyperplasia, some glomerular compensatory appeared hypertrophy, some tubular compensatory appeared expansion.4. Experimental group's spleen became loose capsule, the spleen cord anastomosis were not significantly, some of the splenic nodule were arranged loosely, only the central artery got observed. The red pulp distribution become loose significantly, white pulp's central artery wall got thick and luminal became stenosis.5. The intestinal villi did not stand in a line as before. Some of them were shorten, even broken off. Some striated border was broken away. The assimilate cells and the goblet cells did not stand in regulation. 6. Experimental group leydig cell become blurred or even disappear, in seminiferous tubules of spermatogenic cells reduce at all levels. Sperm reduce within the lumen, at the same time occurred a series of pathological changes ,such as a large number of sperm got abnormal, while the extent of damage of the testis on the group C is higher than Group B, showing that the greater dose of passive smoking has a more serious damage to the testis.Conclusion: The Impact of Passive Smoking on respiratory system, digestive system, genitourinary system, immune system organs of mice have greater pathological changes morphologically, providing a theoretical and scientific basis for comprehensive study impact of passive smoking on human health.