The Interaction Between CETP-TaqIB And ApoE Polymorphism And It's Relation To CHD And Stroke

CHAPTER 1Background: Cholesteryl ester transfer protein (CETP) facilitates the exchange of triglycerides and cholesterol esters between lipoprotein particles, which is a key step in reverse cholesterol transport in humans. Genetic variation at the CETP-TaqIB locus is correlated with plasma lipid levels and coronary heart disease(CHD) risk.Objective: This study was designed to reveal the associations of the polymorphism of cholesteryl ester transfer protein with lipoprotein levels and CHD risk.Methods: In this case-control study, CETP-Taqffi polymorphism was examined in 238 patients with CHD and 203 controls. Polymerase chain reaction-restricted fragments length polymorphism was used to determine CETP genotype.Results: 1. In this study, the B1B1 genotype frequency in CHD patients(39.9%) was significantly higher than that in controls(29.6%, P<0.05)2. The B2 homozygotes significantly increased the plasma level of HDL-C(P<0.01) and ApoAl(P<0.05), decreased the level of TG(P<0.05) than the Bl homozygotes.3. The Bl homozygotes was associated with the higher degree of cononary stenosis than theB2 carriers(P<0.05).4. There was not significant association between CETP-TaqlB genotype and the risk of CHD(P=0.187).Conclusion: There is the first observation of CETP-TaqlB polymorphism distribution in Chinese Hans people. The B1B1 genotype frequency of the patients with CHD was significantly higher than controls.There is significant associations of the B2 homozygotes with higher HDL and ApoAl level, lower TG level than the Bl homozygotes. The Bl allele is not an independent risk factor for CHD.