Chinese Han Population Cetp Gene Multi-state And Coronary Heart Disease And High-density Lipoprotein Cholesterol Associated With Research

Background: Cholesteryl ester transfer protein (CETP) plays a critical role in the process called "reverse cholesterol transport"(RCT), in which the CETP mediated the transfer of cholesterol ester (CE) from high density lipoprotein (HDL-C) to apoB-containing lipoprotein in exchange for triglyceride. Therefore , the CETP regulates serum HDL-C levels and compositions through remodeling HDL particles. Identification of polymorphisms in the CETP gene has given rise to the hypothesis that the CETP deficiency caused by CETP gene mutations could determine serum HDL-C levels and the risk of CHD in general population.Method: a case-control design was applied in this study. In brief, 801 unrelated CHD patients were enrolled from hospitalized patients of Fu Wai Hospital and Cardiovascular Institute between October 1997 and September 2000 who met the following criteria: prior suffered from acute myocardial infarction ; silent myocardial infarction because of temporal changes in electrocardiogram diagnosis ,or documented by coronary angiography at least a 70% stenosis in a major epicardial artery. 818 subjects, age- and sex-matched, were randomly recruited from individuals participating in a community-based survey of cardiovascular risk factors in Beijing. A detail of questionnaires about the risk factors of cardiovascular disease was available and physical examination was carried out. We initially selected 48 unrelated individuals from our case group to detect all polymorphisms of the CETP gene by directly sequencing of genomic DNAs. This sample size provided 95% power to detect alleles with a frequency >1%. Four polymorphisms were selected for genotyping by restriction fragment length polymorphism (RFLP) analysis in all 1619 subjects. To assess the differences for lipoprotein values between the different genotype groups in control, one-way analysis of variance (ANOVA) was performed. Multivariate analysis was performed to investigate the independent effect or interaction between the polymorphisms and serum HDL-C levels or CHD status. All computations were carried out with SAS software. Haplotype frequencies were estimated with the EH program. Pairwise linkage disequilibrium (LD) coefficients...