Studies On The Correlation CETP TaqIB And I405V Polymorphism And Blood Lipid And Coronary Artery Disease

Objective: To investigate the relationship between CETP Taq IB and I405 V polymorphism, lipid metabolism, and coronary heart disease(CHD) among the Chinese Han population of Tianjin area; Especially investigate the relationship between CETP Taq IB polymorphism and coronaty artery disease.Methods: 1.Select 430 patients who were admitted to coronary angiography in Tianjin Chest Hospital from Sep 2012 to Sep 2013.According to the results of coronary angiography classifide into CHD group(n=322) and normal control group(n=108). 2.After admission test total cholesterol(CHO),triglyceride(TG), high density lipoprotein(HDL-C), low density lipoprotein(LDL-C), apolipoprotein A(Apo A1), apolipoprotein B(Apo B). PCR-RELP was executed to determine the genotypes of CETP Taq IB and I405 V. According to the results to investigate the relationship between CETP Taq IB and I405 V polymorphism, lipid metabolism, and CHD.Results: 1.CETP Taq IB genotype frequencies were: B1B1, 36.51%; B1B2, 44.65%; B2B2 18.84%. CETP I405 V genotype frequencies were: II, 25.81%; IV, 66.74%; VV, 7.45%. CETP gene Taq IB sites B2 allele and I405 V sites V allele frequency are 0.412 and 0.408,basically similar to the B2 allele frequencies at home and abroad(B2 0.34~0.46)(V 0.32~0.49). 2. There was no difference among HDL –C level in CHD group genotype B1B1、BIB2 and B2B2(P >0.05). There was no difference among the level of lipid in the control group genotype(P >0.05).There was no difference from serum lipoprotein levels for the three genetic subgroups(P>0.05). In CHD group genotype II had lower HDL cholesterol(HDL-C) levels(1.07±0.27mmol/L) compared with IV(1.08±0.27mmol/L) and VV(1.34±0.31mmol/L), and it reach statistical significance(P<0.01). There was significant difference among the level of HDL-C in the control group genotype(P<0.01).There were not significantly different from serum lipoprotein levels for the three genetic subgroups(P>0.05).3. There were not significant differences in frequencies of CETP Taq IB genotypes and allele between the CHD group and the normal control group. There was not relationship between CETP Taq IB polymorphism and coronary artery stenosis degree(P<0.05). There were not significant differences in frequencies of CETP I405 V genotypes and allele between the CHD group and the normal control group(P>0.05). There was relationship between CETP I405 V polymorphism and coronary artery stenosis degree(P<0.01). 4. Sex, age, hypertension, diabetis mellitus and HDL-C levels were the significant determinant of CHD. Sex, age, hypertension, diabetis mellitus were the risk factor of CHD. HDL-C levels was the protection factor of CHD.Conclusion: 1.The frequencies of CETP Taq IB and I405 V genotype and allele among the population were not different from the other Chinese people and the abroad people. 2.In CHD group genotype B1B1 had lower HDL cholesterol(HDL-C) levels compared with B1B2 and B2B2,but it did not reach statistical significance.B1B2 had higher HDL-C levels than B1B1. In CHD group genotype II had lower HDL cholesterol(HDL-C) levels compared with IVand VV, and it reach statistical significance. 3. There were not significant differences in frequencies of CETP Taq IB and I405 V genotypes and allele between the CHD group and control group. There was not relationship between CETP Taq IB polymorphism and coronary artery stenosis degree. There was relationship between CETP I405 V polymorphism and coronary artery stenosis degree. 4. Sex, age, hypertension, diabetis mellitus were the risk factor of CHD. HDL-C levels was the protection factor of CHD.CETP Taq IB and I405 V polymorphism was not the risk factor of CHD. CETP Taq IB and I405 V might be used to predict the therapeutic effects of statin.