Functional Analysis Of The Genes Encoding RNA Binding Protein (Th-cpRBP29) And Na～+/K～+/Cl～--Cotransporter (Ls-Na～+/K～+/Cl～--Cotransporter)

The growth and development of plant are restrictly controlled by its programmed genetic expression. Many environmental and internal factors can influence the process of development and growth of plant by interfering the genes' expression. Genes' expression is a process under strict regulation, which includes multiple regulating steps such as the activation of gene structure, the initiation of transcription, post-transcriptional processes, translation and post-translational processes. Proteins play a vital role in almost all of the regulation processes especially post-transcription process. RNA binding proteins (RBPs) are the key factors that involve in the post-transcriptional regulation. They affect all but every procedure of the mRNA post-transcription process by directly combining RNA or indirectly regulating the functions of other factors. So, RBPs have wide influence on various aspects of plants that include growth, development, propagation, differentiation and the stress responses, which are all regulated geneticly. From this aspect, it is clear that the study of RBPs and RBPs' encoding genes will help to illustrate the mechanisms that plants adopt in growth, development, stress tolerance, and response.Some documented papers have shown that cpRBP29 belongs to a sort of proteins encoded by nuclear genome and locates in chloroplast. This group of proteins are composed of cpRBP28, cpRBP29(a,b), cpRBP31(a,b) and cpRBP33(a,b). They are closely related to the regulation of gene expression of chloroplast genome. In addition, they also play a role in information exchanging and cooperating between nuclear genome and chloroplast genome. Unfortunately, although some work has been done to identify the RNAs that they process, little is known about the specific function of cpRBP29.Plants are inevitable to suffer from environmental stresses during their whole lives. Stresses might impede the normal growth and development of plants and lead to the decrease of products' quantity, quality and economic values. Researches about the mechanism plants respond to stresses are of great importance for people to avoid the adverse effect of stresses on plants and improve the...