| Arabidopsis thaliana is always best model plant for the research of the plant molecular biology, but it is true glycophyte and can unclose less salt-tolerane mechanisms. Recently, Thellungiella halophila(Salt Cress) ,a close-relative of Arabidopsis thaliana, which is been suggested and be developed as a new salt tolerance model plant. Thellungiella halophila is a salt-tolerance crucifer with a short life cycle, small genome , and with high sequence similarity(about 90-95% on cDNA level) ,similar heredity characteristics and growth habits with Arabidopsis thaliana. So it is convenience for T. halophila to benefit from a lot of Arabidopsis thaliana information data. The purpose of my research is to do some explorations on its salt-tolerance mechanism of Thellungiella halophila as model halophyte .1. Gene silence study of SOS1, SOS2, SOS3, p5CS in Thellungiella halophilaRNAi of SOS1, SOS2, SOS3, p5CS in Thellungiella halophila have been carried on by using the technique of post-transcriptional gene silence(PTGS), containing: RNAi of SOS1, SOS2, SOS3, p5CS from Thellungiella halophila have been constructed; ThSOS1-pGSA1252, ThSOS2-pGSA1252, ThSOS3-pGSA1252 and Thp5CS-pGSA1252 have transferred into Agrobacterium GV1101; Using Floral Dip method , the flower of Thellungiella halophila have been transformed and harvest a wealthy of transformed seeds; Screening the transformed seeds by using herbicide Finale and have gotten about 25, 16, 12, 4 Basat-resistance plants of the gene silence of SOS1, SOS2, SOS3, p5CS in Thellungiella halophila respectively; 4 of Basat-resistance plants in the gene silence of SOS1 and 2 of Basat-resistance plants in the gene silence of SOS2 have been confirmed by PCR. The further molecular identification of these Basat-resistance plants are going on . The purpose of the project is to explore the salt-tolerance function and mechanisms of ThSOS1, ThSOS2, THSOS3 and Thp5CS in Thellungiella halophila.2. Expression analysis of salt induce- relative gene between Thellungiella halophila and Arabidopsis thalianaReal-time Quantitative PCR is a new technique with high sensitivity and high speed , which is applied to the research of gene expression analysis.The project have completed to analyze their expression model of about 50 salt induce- related gene in Thellungiella halophila and about 50 salt-induce-related gene in Arabidopsis thaliana by Real-time Quantitative PCR , and have gained some important experiment results.The condition of the following experiment results is : these gene expression level in 2 hours of 200mM NaCl comparing with control(2 hours in H2O).The higher expression genes which are induced by 200mM NaCl, 2hours in Arabidopsis thaliana : ABI1, ABI2, MAP6(At2g43790), DREB, AP2(At2g20880), MYB(At2g46680), MYB(At3g23250),WRKY(Atlg80840), WRKY(At4g18170), Ethylene responsive element binding factor(At1g53170), 9-cis-epoxycarotenoid dioxygenase NCED(At1g30100) increase their expression by 27.3, 8.3, 3.2, 5.7, 68.7, 19.69, 58.1, 12.3, 6.3, 7.76, 124.6 times; AtNHXl, AtNHX3, AtNHX4 and AtNHX6 increase their expression by 2.19, 2.07, 3.6 and 4.8 times; Galactinol synthase(At1g56600), glycine dehydrogenase(At2g26080) and glyceraldehyde-3 -phosphate dehydrogenase(Atlg13440) increase their expression by 6.54, 4.49 and 2.66 times; Putative Fe superoxide dismutase(At4g25100) and glutathione S-transferase(At4g02520) increase their expression by 1.59 , 3.65 times; Heat shock protein(At5g56030) and late embryogenesis abundant protein (At2g35300) increase their expression by 3.28, 9.14 times; Nonspecific lipid-transfer protein precursor(At5g59310) increase their expression by 2.78 times; Plant defensin-fusion protein (At1g55010) increase their expression by 4.96 times.The higher expression genes that are induced by 200mM NaCl, 2hours in Thellungiella halophila : ABI(BQ079252), PP2C(BM985573) and CDPK(BI698436) increase their expression by 6.2, 3.7 and 7.1 times; DREB and putative ethylene responsive element binding factor(AF499716) increase their expression by 4.9, 24.54 times; Galactinol synthase(BI698...
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