| Objective:Observation of Changyuning granules on active ulcerative colitis rat colon matrix metalloproteinase-1(MMP-1),matrix metalloproteinase-2(MMP-2)expression,in order to further elucidate the mechanism of the treatment of active ulcerative colitis with Changyuning granules.Methods:An experimental model of UC rats was made by modified two nitro chlorobenzene and acetic acid composite method.After one week’s adaptive feeding,60 healthy male Wistar rats were randomly divided into blank group,model group,Changyuning granules group and the salad group,15 rats in each group.in addition to the two nitro chlorobenzene acetic acid method was used as the blank control group,and high fat diet was given for ulcerative colitis model rat.After successful replication,the groups were given 28 days of continuous administration.The pathological changes of colonic mucosa were observed by HE staining,and the expressions of MMP-1 and MMP-2 genes in colon mucosa were observed by reverse transcription polymerase chain reaction(RT-PCR).Results:1.Under the colonic mucosa histopathological results:blank group had no obvious abnormalities;model group were observed in colonic mucosa of rats model with scattered form in the ulcer and crypt abscess,goblet cells disappeared,the glands shape irregular arrangement;Changyuning granules group were seen in colonic epithelial cells of mucosa structure integrity,ulcer tissue part has been showing signs of improvement already,shallower and smaller,crypt abscess disappeared,granulocyte infiltration loss;beauty of salad group showed lesion of colon epithelial cells was the basic structure of integrity,reduce the degree of ulcer,has basically disappeared crypt abscess healing,granulocyte infiltration reduced.2.RT-PCR results showed that:Compared with the control group,the expression of MMP-1mRNA in colonic mucosa of rats increased significantly,the difference was statistically significant(P<0.05);compared with the model group,the expression of Changyuning granules group and the expression of salad group in colonic mucosa of rats with MMP-1mRNA decreased significantly,the difference was statistically significant(P<0.05);Changyuning granules group and colon mucosa the rats in group MMP-1mRNA the salad expression showed no significant difference(P>0.05).3.RT-PCR results showed that compared with the control group,the expression of MMP-2mRNA in colonic mucosa of rats increased significantly,the difference was statistically significant(P<0.05);compared with the model group,the expression of Changyuning granules group and the expression of salad group in colonic mucosa of rats with MMP-2mRNA decreased significantly,the difference was statistically significant(P<0.05);Changyuning granules group and colon mucosa the rats in group MMP-2mRNA the salad expression showed no significant difference(P>0.05).Conclusion:1.Changyuning granule can improve the pathological changes of colonic mucosa in rats with ulcerative colitis,and reduce the infiltration of inflammatory cells.2.The expression of MMP-1mRNA and MMP-2mRNA in colon mucosa of rats with ulcerative colitis treated by Changyuning granule decreased significantly,which may be one of the mechanisms of the treatment of ulcerative colitis with Changyuning granule. |
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