Rats Dampness Spleen Animal Model Experimental Study

Objective: To establish the rats model of dampness obstructing spleen-stomach syndrome and its biological characteristics.Methods : To establish the rats model of dampness obstructing spleen-stomach syndrome by taking high dampness, regulating food and control sleeping, Choosing the typical formula Pingwei powder as the treatment medicine; Using quantitative and half- quantitative method to observe the biological characteristics of rats; The gastroenteric motive function is measured according to the movement of semisolid substance; Using ions selecting pole method to mensurate serum electrolyte; Using GOD-PAP method to mensurate glucose; Using Biuret method to mensurate total protein; Using GPO-PAP method to mensurate triglycerin Grease; Using CHOD-PAP method to measure total cholesterol; Using Enzyme Linked Immunity Sorbent Assay to test serum gastrain, Immunity Globin G, Interleukin 6; Using radioimmunoassay to test serum motilin; Using color-comparing method to test the vivid power of Na~+-K~+-ATP enzyme of liver cell; Using HE staining method to observe the morphological changes of spleen, thymus gland and gastroenteric tissue.Result: 1 It is successful to establish the rats model of dampness obstructing spleen-stomach syndrome and their biology characteristics. ① body features: It takes time to form the rats model, and difficult to recover in short time; Growth situation is not good of model rats described as follows: turbid, tarnish, yellow coat, thin, losing weight, slowly growth of the length of body, tail and tail root diameter; poor appetite; less food; yellow and scanty urine, loose stool; distention of lower abdomen, enlarging of index of lower abdomen girth; sag inspirit, slack and droop scrotum; tired; sticky mouth, a little increase of Ph value in mouth; normal or a little change of body temperature; There is obvious change comparing to control group (P<0.05或P<0.01) . ② Gastroenteric function: The bulge of stomach and intestine of model rats is obvious, Empty ability of stomach and driving force of intestine descend, Absorbing function of stomach and intestine are abnormal, The gastroenteric hormone level is disorder, gastroenteric tissue took place pathologic changes, Comparing to the ones of control group, there is great difference (P<0. 05...