The Efficacy Of Qingre Antai Paste In Treating Experimental Gingivitis During Pregnancy In Rats And Its Effect On C-reactive Protei

Objective: In this study,the experimental gingivitis rat animal model was established by suture ligation and high sugar feeding method.The Chinese medicine clearing heat and preventing abortion ointment was applied to pregnancy gingivitis rats.By comparing the expression of Creactive protein(CRP)in rat gingivitis(GCF),the weight and number of newborn rats,to explore the expression of CRP in pregnancy gingivitis,the relationship between pregnancy gingivitis and low birth weight infants,and the efficacy of the ointment.It provides a new idea for the treatment of pregnancy gingivitis.Methods:(1)Decoction of clearing heat and preventing abortion was extracted and concentrated,and then a sustained release agent was prepared with petroleum jelly and lanolin in a certain proportion.(2)Eighteen female SD rats were randomly divided into three groups: blank control group(group A),model group(group B)and traditional Chinese medicine group(group C).The experimental gingivitis rat model in group B and C was established by suture ligature and high sugar feeding feeding.2 weeks later,all rats were impregnated and separated into cages until delivery.The weight and number of newborn rats were recorded.CRP in rats’ GCF was detected after 1 week of adaptive feeding,after successful modeling and after delivery.Results:(1)The average litter weight and number of newborn SD rats in the three groups were not different;The body weight of newborn rats in group A and group C was higher than that in group B(P < 0.05).(2)There was no significant difference in CRP level in GCF among the three groups after adaptive feeding;The CRP level of group B and C was significantly higher than that of group A(P < 0.05),and there was no statistical difference between group B and group C.The CRP level after delivery showed that group B was significantly higher than group A and B(P <0.05),and there was no statistical difference between group A and group C.(3)There was no significant difference in CRP level in group A at three stages.The CRP level of group B showed that the CRP expression after modeling was significantly higher than that after adaptive feeding(P< 0.05),and the CRP expression after delivery was significantly higher than that after modeling(P< 0.05).CRP level in group C showed that the expression of CRP after modeling was significantly higher than that after adaptive feeding and delivery(P < 0.05),but there was no statistical difference between the levels detected after adaptive feeding and delivery.Conclusion:(1)CRP was highly expressed in pregnant mice with pregnancy gingivitis,further verifying that CRP is an effective indicator of pregnancy gingivitis.(2)The higher the CRP level in rats’ GCF,the lower the weight of newborn rats,suggesting that pregnancy gingivitis may be one of the causes of low birth weight infants.(3)Clearing heat and preventing abortion ointment can effectively reduce CRP level and the incidence of low birth weight infants,and is expected to become a new method for the treatment of pregnancy gingivitis.