| Objectives To investigate the expression and clinical significance of micro RNA-223（Mi R-223）,Forkhead box protein O3a（FOXO3a）and Bcl-2 interacting mediator of cell death（Bim）in the placenta and plasma of patients with preeclampsia（PE）.Methods Strictly follow the inclusion and exclusion criteria,60 cases of pregnant women who delivered by caesarean section from January 2021 to May 2021 in the obstetrics department of Tangshan Maternal and Child Health Hospital were selected as research objects.According to the situation of the study,they were separated into the normal pregnancy group,preeclampsia group（PE group）and severe preeclampsia group（severe PE group）,with 20 patients in each group.Placental tissue and plasma samples were taken and stored according to the requirements of the experiment.Observation of placental tissue morphology using HE staining;The expression of miR-223 in placental tissue and plasma in normal group,PE group and severe PE group was detected by real-time PCR method,and correlation analysis was performed.Bioinformatics predicted FOXO3 as a miR-223 target,and the expression of FOXO3 and BCL2L11 in the normal group,PE group and severe PE group was detected by real-time quantitative q RT-PCR method.The expression of miR-223 and FOXO3 in placental tissues was analyzed for correlation,the expression of FOXO3a and Bim in the placental tissues of three groups was detected by immunohistochemistry and Western Blotting methods,the expression of FOXO3a and Bim in the plasma of three groups was detected by enzyme-linked immunosorbent assay.SPSS 22.0 statistical software was used for data analysis,Image J was used for immunohistochemistry and Western-Blotting band analysis,and Prism 8.0software was used for statistical mapping.Results 1 The placental tissue in the severe preeclampsia group was significantly changed compared with the normal group and the preeclampsia group,the placental trophoblastic cell proliferation and aggregation,the syncytial trophoblast budding,increased nodules,villous fibrin-like necrosis,and increased blood vessels;2 Mi R-223 is expressed in three groups of placental tissues as 0.98（0.96,1.03）,0.45（0.42,0.51）,0.43（0.37,0.47）,the expression of the miR-223 in the placental tissue of the preeclampsia group and the severe preeclampsia group showed a downward trend then the normal group（H=40.23,P<0.001）,but the difference between the preeclampsia group and the severe preeclampsia group was not statistically significant（P=0.179）;miR-223 was expressed in three groups of plasma as 0.96（0.89,1.14）,0.41（0.23,0.47）,0.42（0.26,0.51）.The expression of miR-223 in the plasma group and the severe preeclampsia group was downregulated then the normal group（H=39.50,P<0.001）,but the difference between the PE group and the severe PE group was not statistically significant（P=0.907）;3 There is a positive correlation trend in the expression of miR-223 in placental tissue and plasma（r=0.591,P<0.001）;4 FOXO3 was expressed in three groups of placental tissues as（0.99±0.09）,（0.25±0.07）,（0.42±0.10）,and BCL2L11 was expressed in three groups of placental tissues（1.08±0.19）,（0.34±0.05）,（0.43±0.06）,respectively.The expression of FOXO3 and BCL2L11 in placental tissue in the preeclampsia group and the severe preeclampsia group showed a decreasing trend then the normal group（all P<0.001）.5Mi R-223 in placental tissue is positively correlated with the expression of FOXO3（r=0.619,P<0.001）;6 The expression of FOXO3a in the three groups of placental tissues was（1.30±0.27）,（0.87±0.09）,（0.54±0.11）,and the expression of Bim in the three groups of placental tissues was（1.24±0.12）,（0.85±0.15）,（0.53±0.15）.Compared with the normal group,the protein expression of FOXO3a and Bim in the preeclampsia group and the severe preeclampsia group showed a downward trend（all P<0.001）.7 The results of immunohistochemistry showed that FOXO3a was mainly localized in the nuclei of trophoblasts and mesenchymal stem cells,and the relative expression of FOXO3a in the three groups of placental tissues was（0.96±0.07）,（0.68±0.07）,（0.44±0.06）,and the expression of FOXO3a in the PE group and the severe PE group showed a decreasing trend compared with the normal group（F=343.223,P<0.001）;Bim is mainly localized in trophoblast cytoplasm,and the relative expression in the three groups of placental tissues is（0.95±0.06）,（0.67±0.05）,（0.56±0.05）,compared with the normal group,the expression of the preeclampsia group and the severe preeclampsia group showed a decreasing trend（F=277.016,P<0.001）;8 The expression of FOXO3a in the plasma of the three groups was 821.29（770.74,876.42）,940.78（784.05,982.10）,937.05（847.13,1011.17）,and the expression of Bim protein in the plasma of the three groups was 2052.28（1919.09,2136.27）,2241.57（2149.71,2331.13）,respectively.2210.58（2158.17,2400.79）,Compared with the normal group,the expression of FOXO3a and Bim in the preeclampsia and severe preeclampsia groups in plasma showed an increase trend（all P<0.001）,but the expression differences between FOXO3a and Bim in the preeclampsia group and the severe preeclampsia group were not statistically significant(PFOXO3a=0.450,PBim=0.476).Conclusions 1 The expression of miR-223 in placental tissue and plasma in patients with preeclampsia was down-regulated,and the two were positively correlated;2 The expression of FOXO3a,and Bim in the placental tissue of patients with preeclampsia was all down-regulated,which was related to the severity of the disease;3 The differential expression relationships and related pathways of miR-223,FOXO3a and Bim in placental tissue and plasma in patients with preeclampsia may play an important role in the pathogenesis of preeclampsia.Figure12;Table23;Reference 111... |
