Study On The Changes Of Lymphocyte Subsets In Hospitalized Patients With Blood Diseases

Objective:To explore the change characteristics of lymphocyte subsets in patients with common blood diseases,in order to provide an important basis for diagnosis and prognosis evaluation of patients with blood diseases.Method:Through retrospective study,93 newly diagnosed inpatients with blood diseases in the 967 Hospital of the Chinese People’s Liberation Army were retrospectively investigated,and T,B,NK lymphocyte subsets were taken as the entry point to analyze the change characteristics of lymphocyte subsets of different types of blood system diseases.Result:1.The abnormal detection rate of TLC in 93 patients with hematologic diseases was47.3%,and the detection value below the lower limit of normal reference value was the most common.2.The abnormal detection rates of CD3~+,CD4~+,CD3~+%,CD4~+%and CD4~+/CD8~+in peripheral blood T lymphocyte subsets were 39.8%,48.4%,19.4%,31.2%and 19.4%,respectively,among which abnormal detection values below the lower limit of normal reference values were the most common.The abnormal detection rates of CD8~+and CD8~+%were 36.5%and 23.7%,respectively,and the detection values were higher than the upper limit of normal reference value.The abnormal detection rates of CD19~+and CD19~+%in B lymphocyte subsets were 58.1%and 51.5%,respectively,and the detection values were higher than the upper limit of the normal reference value.The abnormal detection rates of CD16~+CD56~+and CD16~+CD56~+%in NK lymphocyte subpopulation were 34.5%and29.0%,respectively,and the detection values below the lower limit of normal reference values were the most common.3.According to the groups of affected hemocytes,the median TLC value of PLT group was significantly higher than that of RBC group and WBC group,and the difference was statistically significant compared with WBC group(P<0.05).The abnormal detection rate of WBC group was the highest,reaching 80.8%,followed by RBC group.The difference between the two groups and PLT group was statistically significant(P<0.05).4.In T lymphocyte subsets,the median values of CD3~+,CD4~+and CD8~+in PLT group were higher than those in the other two groups,but only the median values of CD3~+and CD4~+in PLT group were statistically significant compared with those in WBC group(P<0.05).The abnormal detection rates of all indexes in WBC group were higher than those in the other two groups,and the abnormal detection rates of CD3~+and CD4~+%were significantly different from those in PLT group and RBC group,respectively(P<0.05).5.In B lymphocyte subsets,the median value of CD19~+detected in PLT group was the highest,and the difference between PLT group and WBC group was statistic-ally significant(P<0.05).Among the abnormal CD19~+detection values in the three groups,the WBC group was mostly lower than the lower limit of the normal reference value,while the RBC group and the PLT group were mostly higher than the upper limit of the normal reference value.The abnormal detection value of CD19~+%in the three groups was higher than the upper limit of the normal reference value.6.In NK cell subsets,the median values of CD16~+CD56~+and CD16~+CD56~+%in PLT group were higher than those in RBC group and WBC group,but there was no statistical significance among all groups(P>0.05).The abnormal detection rate of CD16~+CD56~+in RBC group was higher than that in the other two groups,and the abnormal detection rate of CD16~+CD56~+%in WBC group was higher than that in the other two groups,but there was no statistical significance among all groups(P>0.05).Conclusion:1.In this study,immune dysfunction was common in the patients with blood diseases,especially immunosuppression.The abnormal cellular immune function was characterized by a decrease in the number and proportion of CD4~+cells,an increase in the number and proportion of CD8~+cells and a decrease in the ratio of CD4~+/CD8~+.The abnormal humoral immune function is characterized by increased number and pro-portion of CD19~+cells.Abnormal immune function of natural killer was characterized by decreased number and proportion of CD16~+CD56~+cells.2.After classification according to the affected blood cell lines,patients can still show different degrees of abnormal immune function changes,most of which are manifested as reduced the number of TLC,among which the immune function abnormalities are more common in the patients with leukocyte diseases,which are manifested as reduced cellular immunity,humoral immunity and natural killer immunity.The abnormal immune function in the patients with red cell disease was manifested as hyper-cellular immunity,hyper-humoral immunity and decreased natural killer immunity.The abnormal immune function in patients with platelet disease is manifested as reduced cellular immunity,humoral immunity,and natural killer immunity.