Expression And Clinical Analysis Of Peripheral Blood T Lymphocyte Subsets,Plasma MiRNA-223,and Inflammatory Markers In Patients With Non Segmental Vitiligo

Objective:To research the change in the expression of peripheral blood lymphocyte subsets,plasma mi RNA-223 and related inflammatory indicators in patients with non-segmental vitiligo,and to explore its role in disease activity monitoring and disease evaluation of vitiligo.Methods:1.From March 2022 to December 2022,the fasting venous blood of 60 patients with non-segmental vitiligo diagnosed by our department(30 patients with progressive and 30 patients with stable non-segmental vitiligo)and 30 healthy controls were collected,and the general conditions of the patients(gender,age,skin lesion area,VIDA score,disease severity grading and family history,etc.)were collected.2.Flow cytometry was used to detect the differences in the counts of CD3~+T cells,CD4~+T cells,CD8~+T cells count,and CD4~+/CD8~+T ratios in the target study population,and to compare the differences in the above indicators among different groups.3.The expression level of mi RNA-223 in the target study population was detected by reverse transcription fluorescence quantitative PCR,and the changes of different grouping indicators were compared.4.Collect the levels of HDL cholesterol ester and blood routine in the target study population,and compare the changes of monocyte to specific HDL cholesterol ratio,monocyte to specific lymphocyte ratio,neutrophil to lymphocyte ratio,platelet count to lymphocyte ratio and mean platelet volume in contrasting groups.Result:1.Peripheral blood T lymphocyte subsets:Compared with the contrasting group,the CD3~+T cells(P<0.05),CD4~+T cell count(P<0.001)and CD4~+/CD8~+T ratio in the non-segmental vitiligo group were significantly lower(P<0.001),and the CD8~+T cell count was significantly higher(P<0.001).The CD3~+T cells(P<0.05),CD4~+T cell count(P<0.001)and CD4~+/CD8~+T ratio in the contrasting group were significantly lower than those in the stable non-segmental vitiligo group(P<0.001),and the CD8~+T cell count was significantly higher(P<0.001).For the non-segmental vitiligo group with different severity,the CD4~+T cell count and CD4~+/CD8~+T ratio in level III group were significantly lower than those in level II group and level I group(P<0.001),and the CD8~+T cell count in level III group was significantly higher than those in level II group and level I group(P<0.001).2.Plasma mi RNA-223 expression level:The plasma mi R-223 expression level in patients with non-segmental vitiligo was actually decreased(P<0.05).The expression level of mi R-223 in plasma of patients with advanced non-segmental vitiligo was significantly lower than that of patients with stable non-segmental vitiligo(P<0.05).However,there was insignificant difference between the non-segmental vitiligo groups with different disease severity(P>0.05).3.Inflammation index:The levels of MHR,MLR,NLR and PLR in non-segmental vitiligo group were necessarily higher(P<0.05).MHR,MLR,NLR and PLR in advanced non-segmental vitiligo group were significantly larger than those in stable non-segmental vitiligo group(P<0.05).In addition,MHR increased with the severity of vitiligo disease.Conclusion:1.Non-segmental vitiligo patients have cellular immune disorders.The CD4~+T cell count and CD4~+/CD8~+ratio are uncorrelated with the severity of the disease,while the CD8~+T cell count is positively correlated,suggesting that lymphocyte subsets can be used as biological indicators of the disease severity grading of non-segmental vitiligo patients.2.The low expression of mi RNA-223 in patients with non-segmental vitiligo suggests that it plays a role in the pathogenesis of vitiligo.The differential expression of mi RNA-223 in patients with non-segmental vitiligo at different stages suggests that mi RNA-223 can be used as a biological marker for the stage of non-segmental vitiligo.3.The inflammatory reaction in patients with non-segmental vitiligo is enhanced,and the monocyte/high-density lipoprotein cholesterol ratio rise with the severity of vitiligo disease,suggesting that it can be used as a biological indicator of disease severity grading in patients with non-segmental vitiligo.