| Part I: Clinical study--Clinical retrospective study on the treatment of novel coronavirus infection with Hejie Shenshi DecoctionObjective: To retrospectively analyze clinical data from 63 critically ill patients at Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology(Optics Valley Hospital)for the purpose of this study,and to explore the clinical effectiveness of Hejie Shenshi Decoction in COVID19 treatment.Method: Based on inclusion and exclusion criteria,63 patients with severe COVID-19 were divided into two groups,the conventional treatment group received conventional treatment(antiviral treatment,symptomatic treatment,nutritional support,etc.),and the decoction group was added along with conventional treatment,and the clinical data between the 2 groups were triaged.Clinical efficacy was evaluated by comparing the length of hospitalization,fever and cough duration,nucleic acid negative time and time of admission and absorption of lung lesions in COVID-19 patients between the two groups.Results: Through statistical analysis,the cough duration of the two groups was significantly different after admission(P < 0.05).The decoction group had a shorter cough duration than the conventional therapy group.The time to onset of lung injury uptake and the time to negative nucleic acid processing were significantly different between the two groups(P < 0.05).The time of onset of lung injury uptake and negative transformation time of nucleic acid in the Hejie Shenshi decoction group were shorter than those in the conventional treatment group.The difference in length of hospital stay between the 2 groups was significant(P < 0.05),and hospital length of stay in the decoction arm was significantly shorter than that in the conventional therapy arm.No patients in the two groups turned into critical patients,and all 63 patients recovered and were discharged from hospital.Conclusion: In the treatment of novel coronavirus infection,the decoction can shorten the duration of clinical symptoms,promote negative nucleic acid conversion,accelerate the absorption of lung lesions,shorten the length of hospital stay,better than the conventional treatment group.Part II: Animal experimental study--Study on the mechanism of treating viral pneumonia with Hejie Shenshi DecoctionObjective: To study the possible mechanism of decoction of Hejie Shenshi in the treatment of viral pneumonia.Methods: Sixty C57BL/6 male mice were randomly divided into normal group,model group,drug-positive group,low-dose Hejie Shenshi decoction group,moderate dose group and high-dose group with 10 mice in each group.Mouse model of viral pneumonia was induced by Poly(I:C).After successful modeling,in the drug-positive group,dexamethasone was administered intragastrically at1.56mg/kg,and the low-dose,moderate-dose and high-dose groups were intragastrically given Hejie Shenshi decoction 10.14,20.28 and 40.56g/kg.Both the normal group and the model group were given 0.9% sodium chloride solution of equal volume.After one week of intervention,the dry/wet weight ratio of the lungs and the protein content of the alveolar lavage solution were measured.HE staining was used to observe histopathologic changes in the lungs,and IL-6,IL-1β,and TNF-α levels in the alveolar lavage solution were detected by ELISA assay.Results: Compared to the model group,airway wall and lung tissue alveolar wall damage was significantly reduced in the Hejie Shenshi decoction group at each dose level.Compared with model group,the dry/wet weight ratio of mice in the Hejie Shenshi decoction each group was elevated(P<0.05),there was a decrease in the levels of IL-6,IL-1β and TNF-α in the alveolar lavage fluid of mice in the Hejie Shenshi decoction group(P<0.05),and protein content in alveolar lavage fluid from mice in both the moderate dose and high dose groups was decreased(P<0.05).And it is dose-dependent.Conclusion: The Hejie Shenshi decoction can effectively ameliorate lung injury in the mouse model of viral pneumonia,and its mechanism may be linked to reduced inflammation and reduced viral mucus secretion. |
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