The Up-Regulation Of Intestinal SGLT1 Expression After Sleeve Gastrectomyinterferes With The Effect Of Metabolic Surgery

Objective:Sleeve gastrectomy(SG)has been widely used in the treatment of obesity due to its significant weight loss and anti-diabetic effects,but there are still cases of long-term weight loss failure.Our aim was to explore the mechanism of the sleeve stomach on weight loss in mice to understand how the initial improvement in glucose metabolism occurs.Methods:C57/BL6 mice were randomly divided into two groups,one group was sleeve stomach group,the other group was sham operation group.Body weight and blood glucose levels were detected at 2 weeks,1 month and 2 months after surgery,and the changes of intestinal villi were detected by HE staining.The expression of sodium-glucose cotransporter 1(SGLT1)in each intestinal section was detected by POLYMERase chain reaction(PCR).Results:SG mice reduced food intake and lost weight within 30 days after surgery,exceeding those in the sham group after 30 days.Polymerase chain reaction test showed that SGLT1 expression decreased 1 month after SG and increased 2 months after SG.Although the initial level of SGLT1 expression in the stomach was much lower than in the intestine,it first increased and then decreased after surgery.Conclusions:SG may improve metabolism by decreasing the expression of SGLT1.In addition,the blood glucose rebound after SG may be the result of the upregulation of SGLT1.