| Part I Animal Model of the Sleeve gastrectomy on the Diet-Induced obese Rats Objective:build up a stable animal model of the Sleeve gastrectomy (SG) on the Diet-Induced obese RatsMethods:Induced by high fat diet (HFD), the obese SD rats were ramdomly distributed to experimental group and control group; those in the experimental group were treated with SG, for those in the control group, sham-surgery were applied. After 8 weeks, differences of the post-operation weight loss of the body and fat content were measured between the 2 groupsResults:58% of total rats were successfully induced to obesity by HFD; the experimental group exhibited a 3-week consecutive weight-loss for 13.25% by the end of the 3rd week, the control group on the other hand, presented 1-week weight loss,(3.84%); after 3 weeks, the experimental group had a slow rebounce of weight, a mean 1.40±0.32g per day, by the end of 8th week, the weight was still significantly lower than the pre-operation; compared to the control group, which showed a notable weight gain for 1.63±0.23g per day, an increased weight than before; the amount of diet intake in the experimental group decreased compared to pre-operation(p<0.05), the control group exhibited no significant change (p>0.05); subcutaneous fat contents and deep deposit of omental fat pad of the experiment group were significantly lower than the control group's (p<0.05)Conclusion:After SG, there was a significant decrease of weight, abdominal fat and diet intake, this could establish a stable animal model for the study of bariatric surgery approach. Part 2:Effect and mechanism of Sleeve gastrectomy on weight loss.Objective:explore the effect and mechanism of Sleeve gastrectomy on weight loss. Methods:The HFD induced obese rates were distributed to SG group, sham-surgery pair-fed (SSPF) group and sham-sugery (SS) group. After 8-week of consecutive observation, comparisons were scaled in weight loss, diet intake, differences in fast concentration of Ghrelin,GLP-1,PYY3-36.Results:SG group presented significant decrease of diet intake,3 consecutive week of weight loss, then followed a minor rebounce, yet still lower than before by the end of 8th week, the concentration of Ghrelin in the peripheral blood decreased, while GLP-1,PYY3-36 increased, with the prolonged time span, Ghrelin exhibited a gradual increased concentrate, and GLP-1,PYY3.36 decreased on the contrary; SSPF group shared a similar trend in weight change, yet a minor decrease, and a more significant weight gain, the Ghrelin,GLP-1,PYY3-36. showed no change; SS group showed no significant change in diet intake, Ghrelin,GLP-1,PYY3-36., after 8 weeks, the body weight was higher than before(P<0.05)Conclusion:The physical restriction because of the decreased volume due to SG may not be the only reason that lead to reduced diet intake and weight loss; the SG could change the secretion of gastrointestinal hormone; after SG, the decreased Ghrelin and the increased GLP-1 PYY3-36 play a crucial role in weight loss. Objective:Explore the effect and mechanisms in relieving type 2 diabetes mellitus after Sleeve GastrectomyMethods:To establish a type 2 diabetes mellitus animal model, the obese rats were injected with STZ, then followed to distribution into SG group, sham-surgery pair-fed (SSPF) group and sham-surgery (SS) group. After 8-week of consecutive observation, comparisons were scaled in weight loss, diet intake, differences in fast glucose, insulin, insulin resistance index and concentration of Ghrelin,GLP-1,PYY3-36.Results:SG group presented significant decrease of diet intake,3 consecutive week of weight loss, then followed a minor rebounce, yet still lower than before by the end of 8th week, plasma insulin decreased, the insulin sensitivity index increased, the concentration of Ghrelin in the peripheral blood decreased, while GLP-1,PYY3-36 increased, with the prolonged time span, Ghrelin exhibited a gradual increased concentrate, and insulin sensitivity index GLP-1,PYY3-36 decreased on the contrary; SSPF group shared a similar trend in weight change, yet a minor decrease, and a more significant weight gain(P<0.05), the insulin Ghrelin,GLP-1,PYY3-36. showed no change, insulin sensitivity index increases but without statistic significance (P>0.05); SS group showed no significant change in diet intake, insulin, insulin sensitivity index, Ghrelin,GLP-1,PYY3-36., after 8 weeks, the body weight was higher than before(P<0.05)Conclusions:the relieve of blood glucose is prior to the weight loss, which suggest the reduced diet intake is not the only reason that lead to it; after SG, the decreased Ghrelin and the increased GLP-1 PYY3-36 could be the mechanisms for relieving type 2 diabetes, meanwhile the plasma insulin concentration decrease, the sensitivity of insulin improves.
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