Effect Of Weight Loss And Lipid Metabolism Submitted To Sleeve Gastrectomy Modified Plus Jejuno-ileal Bypass

Part I Influence of Sleeve Gastrectomy on Several Metabolic Experimental ModelsObjective:To explore the effect of sleeve gastrectomy on glycometabolism and lipid metabolism of several experimental metabolic models. Methods:high-fat diet induced obesity rats(F group) and GK rats (D group)and Non-obese Wistar rats(C group) were underwent sleeve gastrectomy(SG) and comparing weight reduction,food-intake,active plasma ghrelin, insulin, active plasma GLP-1,glycemia and plasma triglyceride between three groups.Results:F and D animals showed effective weight loss and anorexic effects during eight postoperative week (P<0.05), F rats showed more significant weight reduction. Body weight of F rats returned to the pre-operative level at postoperative 6 weeks,while body weight of the D rats returned to the pre-operative level 4 weeks after ooperation. Weight gain of the F and D rats was slower than C rats (P<0.05).Foodintake of F rats decreased during postoperative 4 weeks, Foodintake of D rats decreased during postoperative 3 weeks,compared to C rats, Foodintake of F and D rats decreased more significantly.Comparing to C rats,the rats in F and D group exhibited significantly decreased levels of plasma ghrelin and elevated levels of plasma GLP-1 (P<0.05);D rats showed improvent in glycemia. Insulin concentrations were significantly increased after operation in D and F rats (P<0.05).F group showed elevated plasma HDL level after operation,as though they don not achieved significant(P>0.05).Conclusion:Our data demonstrated that SG could represent an effective way of improvement glycometabolism and lipid metabolism on several experimental metabolic models.Part 2:The Effects of Sleeve Gastrectomy Surgery with Modified Jejuno-Ileal Bypass on the Body Weight, Food Intake and the LevelsObjective:The objective of this study is to evaluate the effects of a combined surgery of sleeve gastrectomy (SG) and modified jejunoileal bypass (SG-JIB) on the body weight, food intake and the plasma levels of active glucagon-like peptide-1 (GLP-1) as well as total ghrelin of rats.Methods:Rats were subjected to SG-JIB(n=12), SG (n=12);JIB(n=12), sham surgery ad lib-fed (n=10). SG-JIB was performed with sleeve gastrectomy and end to side anastomosis of part of jejunum (25 cm from the ligament of Treitz) to the ileum 25 cm proximal to the cecum.The body weight and food intake were evaluated during 10 consecutive weeks postoperatively. The levels of active GLP-1 and total ghrelin in plasma of the rats were measured by ELISA assay.Results:SG-JIB treated rats produced a sustained reduction in food intake and weight gain than that of SG and JIB. SG-JIB was associated with elevated plasma active GLP-1 and decreased total ghrelin concentrations.Conclusion:These data demonstrated that SG-JIB could efficiently reduce the body weight, food intake as well as alter obesity-related hormone levels of the rats, indicating that SG-JIB could be potentially used for the treatment of obesity. Part3:Lipid Metabolism Submitted to Sleeve Gastrectomy Plus Modified Jejuno-Ileal BypassObjective:Obesity is associated with fat accumulation and lipid metabolism disorders. The purpose of this study is to evaluate the effects of sleeve gastrectomy plus modified jejuno-ileal bypass (SG-JIB) on the and lipid metabolism in peripheral tissues.Methods:Fat diet-induced 48 obese rats were subjected to SG-JIB (n=12) and sham operation (SO)and pair-fed (n=12). Sham operation (SO)were included SO-HF rats which delivered the same high fat diet as the surgery group,and SO-SD rats which delivered the standerd food.Foodintake and body weight were evaluated during consecutive 4 postoperative weeks.fat mass and lean body mass,Hypothalamic growth hormone secretagogue receptor 1 (GHSR-1) in hypothalamus, gene involved in then regulation of lipogenesis and lipid oxidation in white adipose tissue(WAT), liver and skeletal muscle were investigated at 4 weeks after operation.Results:Compared to SO and PF rats,SG-JIB rats showed susstained weight loss and anorexia effects 4 weeks after operation. SG-JIB produced a decreased lipogenesis activity in WAT liver and skeletal muscle when compared to SO and PF rats, fat mass and lean body mass were significant decreased. The treatment of SG-JIB led to elevated GHSR-1 in hypothalamus.Conclusions:These data demonstrated that SG-JIB could influenceed lipogenesis activity in white adipose tissue, liver and skeletal muscle. Elevated GHSR-1 in hypothalamus may connect to changes in lipid management in tissues.