Studies On The Molecular Mechanisms Of KGA Regulating Cell Apoptosis In Non-Small Cell Lung Cancer

Glutamine metabolism plays an important role in the occurrence and development of tumors,among which glutaminase is the key rate limiting enzyme of glutamine metabolism.In human body,glutaminase can be divided into kidney type glutaminase(GLS)and liver type glutaminase(GLS2).Among them,kidney type glutaminase GLS functions as a tumor promoting gene.GLS has three subtype: kidney type glutaminase(KGA)、glutaminase C(GAC)and GAM.Until now,the studies on the functions of GLS in tumor mainly focused on GAC,but little is known about the function of KGA and there is no study of KGA in non-small cell lung cancer(NSCLC).In this study,we found that KGA was highly expressed in both non-small cell lung cancer(NSCLC)cells and tissues.Overexpression of KGA significantly promoted proliferation,migration and clonal colony formation of NSCLC.Further studies showed that KGA could inhibit apoptosis in NSCLC cells.Further mechanism research shows that KGA interacted with methionine adenosine transferase MAT2 A,which promoted the expression of KGA.Meanwhile,we also found that MAT2 A could inhibit apoptosis by promoting the expression of KGA.Therefore,our study clarified the cancer promoting function of KGA in non-small cell lung cancer cells for the first time,revealed the important role of KGA in the process of apoptosis,which showed a new idea for people to deeply understand the role of KGA in the process of carcinogenesis and development,and provided a theoretical basis for tumor treatment strategies by targeting glutamine metabolism.