| The purpose of this experiment is to determine the effect of Clostridium butyricum on broiler production,clarify the mechanism of Clostridium butyricum promoting absorption,improving intestinal health and its replacement effect by studying the effects of Clostridium butyricum on broiler growth performance,slaughtering performance,economic benefits,intestinal development and morphology,nutrient and amino acid utilization.A total of 750 healthy one-day-old Ross 308 commercial broilers(30 as metabolic endogenous group and standby chicken)were randomly divided into 3 treatments that consisted of 8 replicates with 30 broils each.The dietary treatments were:(1)treatment 1: In the control group,the basic conventional non-resistant diet for broilers(NY2004 recommended nutrition level),except amino acids,vitamins,trace elements and enzyme preparations that meet the nutritional requirements of broilers,does not add any microecological agents and other additives;(2)treatment 2: on the basis of treatment 1,75 g/t of broad-spectrum antibiotics(aureomycin)were added to the diet;(3)treatment 3 added 50 g/t of Clostridium butyricum on the basis of treatment 1.The test was carried out in two stages: starter period(1-21 d)and finisher period(22-42 d).The results showed that: 1)On day 21 and 42,the body weight was significantly increased for the diet with antibiotics and Clostridium butyricum as compared with the control group(P< 0.05).In comparison to the control,the diet with antibiotics and Clostridium butyricum significantly increased the average daily weight gain in days 1-21,22-42 and1-42 days(P <0.05).However,it decreased the average daily feed intake(P < 0.05)and the feed weight ratio in days 22-42 and 1-42(P < 0.05).Furthermore,it significantly reduced the feed weight ratio(P < 0.05)in the days 1-21 stage,and improved the European benefit index(P < 0.05).But there was no significant difference between the Clostridium butyricum group and the antibiotic group.The results of the groups with antibiotics and Clostridium butyricum can improve the feed conversion efficiency of commercial broilers,reduce feed consumption and feed costs,and increase the market weight,which can increase the economic benefits by 0.75 yuan/chicken and 0.64 yuan/chicken respectively.2)The addition of antibiotics and Clostridium butyricum in the diet could significantly increase the semi-clean bore rate and reduce the abdominal fat rate of commercial broilers(P <0.05)on day 21.These two treatments also increased the slaughter rate,semi-clean bore rate and full clean bore rate apparently on day 42(P < 0.05)and the index of spleen,thymus and the bursa of fabricius on day 42(P < 0.05).Besides,the abdominal fat rate of the clostridium butyrate group on day 21 was significantly lower than that of the control group and the antibiotic group(P < 0.05),and the index of spleen,thymus of the Clostridium butyricum group on day42 were significantly higher than that of the antibiotic group(P < 0.05).However,there was no significant difference in other slaughter performance and immune organ index.3)The addition of antibiotics and Clostridium butyricum to the diet could significantly increase the total length of the digestive tract,the relative weight of the small intestine and the total relative weight of the digestive tract at 21 day(P < 0.05).Compared to the control group,the small intestine weight of the Clostridium butyrate group was significantly increased(P <0.05).These treatments also increased the total weight of small intestine and digestive tract of commercial broilers at 42 day(P < 0.05),and increased the length of small intestine as well as the total length of digestive tract,and tended to improve at 21 day and 42 day(0.05 < P < 0.10).However,other digestive tract indicators had no significant impact.The addition of antibiotics and Clostridium butyricum in the diet could significantly reduce duodenal injury at 21 day(P< 0.05),and the injury to duodenal,jejunal and ileal at 42 day(P < 0.05).The addition of antibiotics and Clostridium butyricum in the diet could significantly increase the villus length and gland ratio of the jejunum and ileum of commercial broilers on day 21(P < 0.05).In addition,there was a trend to increase the villus length and gland ratio of the duodenum at 21day(0.05 < P < 0.10).4)The addition of antibiotics and Clostridium butyricum in the diet significantly improved the apparent utilization and the true utilization of dry matter(DM),crude protein(CP)and total energy(GE)of commercial broilers(P < 0.05),.And the results of these group have a trend to improve the apparent utilization and true utilization of organic matter(OM)and crude fat(EE)(0.05 < P < 0.10).The addition of antibiotics and Clostridium butyricum in the diet significantly increased the apparent and true utilization of isoleucine,histidine,leucine,threonine and total essential amino acids,the apparent utilization of lysine and valine(P < 0.05),and had a trend to increase the apparent and true utilization of phenylalanine and the real utilization rate of valine(0.05 < P < 0.10).The addition of antibiotics and Clostridium butyricum in the diet significantly increased the apparent and true utilization of tyrosine,serine,alanine and total non-essential amino acids(P < 0.05),and had a trend to improve the apparent and true utilization of cystine and the apparent utilization rate of aspartic acid and proline(0.05 < P <0.10).In conclusion,Clostridium butyricum can promote intestinal development,improve intestinal health,production performance and slaughter performance of commercial broilers.Besides,clostridium butyricum addition elevates the nutrient and amino acid utilization.This function is similar to the effects of antibiotics,which indicated that Clostridium butyricum can substitute for antibiotics to some extent. |
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