Synthesis Of Novel Mannose Polymers Via Click Chemistry And ROMP Polymerization And Their Specific Recognition Performance

More and more glycopolymers have been proven to mimic the"sugar cluster effect"of natural sugars and produce specific multivalent interactions with specific lectins on cells.Among them,mannose residues and Con A have this special mutual recognition ability.In order to further study the improvement of the structure and properties of sugar-containing polymers,scientists began to pay attention to the structural functionalization of sugar-containing derivatives.Explore the reactivity of sugar unit functional groups,the superiority of polymerization methods,and more.The preparation of a series of glycopolymers with a clear structure and a controllable molecular weight,and the exploration of the structure of glycopolymers that have the best recognition effect with lectins under similar conditions,have always been research hotspots.This is also of great significance to the development of late-stage targeted drugs.In this paper,combined with the research trend of glycopolymers,a series of well-defined and highly ordered functionalized mannose polymers were synthesized by click chemistry(Cu AAC,Thiol-ene)and ring-opening metathesis polymerization(ROMP),including copolymers of sugar units with different thiol derivatives and homopolymers of different sugar units.Hydrogen nuclear magnetic resonance(~1H-NMR)confirmed the structure of the target sugar-containing polymer,and gel permeation chromatography(GPC)showed that a narrow molecular weight distribution width(PDI≤1.40)and a controllable side chain structure were prepared.Sugar-containing linear polymers of heterogeneous/homogeneous sugar units.Further,the turbidimetric method was used to confirm that the preparedα-mannose-containing polymers had strong specific recognition effects with Con A,and thatβ-glucose andβ-galactose could promote the recognition of mannose and ConA.