Synthesis Of Novel Functional Glycopolymers Via RAFT-PPM Strategy And Their Recognition Of Lectin Con A

Glycopolymers are synthetic polymers whose structural side chains contain carbohydrate fractions.In theoretical research,it is used to explore the influencing factors of the recognition affinity of polyvalent sugar clusters and lectins,and in biomedicine,due to its good biocompatibility and hydrophilicity,it is mainly used in drug delivery and cell imaging.In this paper,it is proposed to prepare multifunctional"smart"glycopolymers with p H responsiveness or fluorescence properties by RAFT polymerization and post-polymerization modification,and to study their specific recognition with lectin Con A.The structure and composition of seven homopolymers with relatively narrow molecular weight distributions were characterized by ¹H NMR,¹⁹F NMR and GPC.The p H responsiveness was verified by dynamic light scanning（DLS）,that is,the particle size decreases with the increase of p H value.The binding ability of synthetic homopolymer to lectin Con A was studied by turbidimetric determination and isothermal titration calorimetry（ITC）.The results showed that the polymer containingα-D-mannose and amino acids in the side chain was more recognizable to Con A than the glycopolymer without amino acid modification.The polymer containing heterosugars（α-mannose andα-glucose）has a stronger ability to recognize lectins than the conogenic sugar polymers（α-mannose andα-glucose）.In addition,in the fluorescence characteristic test of polymer,it was found that lectin Con A had a significant fluorescence quenching effect.These well-structured functional glycopolymers provide a direction for the development of targeted drug carriers.