Preparation Of Phthalocyanine (Naphthalocyanine) Based Nanomaterials And Antibacterial Research Of PDT/PTT

In recent years,due to the abuse of antibiotics,the emergence of drug-resistant bacteria posing a hazard to human health.Compared with traditional antibiotic therapy,phototherapy including photodynamic therapy(PDT)and photothermal therapy(PTT),has the advantages of wide applicability,non-invasive,without side effects and without drug resistance,providing a good treatment strategy for bacterial infection.In this paper,two kind of nanoparticles containing(na)phthalocyanine were prepared,their characterization and PDT/PTT antibacterial studies were carried out:(1)Preparation,characterization and PDT/PTT antibacterial research of bis-(sodium p-phenoxy sulfonate)-silicon naphthocyanine self-assembled nanoparticles.Bis-(sodium p-phenoxy sulfonate)-silicon naphthocyanine(Nc S)was synthesized and characterized by ~1H NMR and MADLI-TOF MS.Nanoparticles with diameters of 20,70,110,and140 nm were obtained by self-assembly of Nc S at 0%,0.4%,1%,and 3%CEL aqueous solutions,which were named NanoNcS20,NanoNcS70,NanoNcS110,and NanoNcS140,respectively.Under 780 nm laser irradiation,the photothermal efficiency of these nanoparticles were 40.00%,21.08%,15.06%and 11.60%,respectively,And the singlet oxygen yields were 20.40%,14.9%,12.9%and 8.2%.The superoxide anion release rate was NanoNcS20>NanoNcS70>NanoNcS110>NanoNcS140,respectively.When the concentration was 12.8 n M,the inhibition efficiency of E.coli was 99.99%,84.89%,84.29%,83.54%and against S.aureus were98.36%,83.24%,82.31%and 77.97%,respectively.Furthermore,the minimum inhibition concentration of NanoNcS20 on E.coli and S.aureus were 0.2 n M.The fluorescence of NanoNcS20 was enhanced after illumination,and the same phenomenon occurred when heated at 60℃,indicating that the fluorescence enhancement was caused by the temperature increase.TEM images showed that NanoNcS20 were disassembled after heating.In vivo of zebrafish,NanoNcS20 also showed enhanced fluorescence after illumination,indicating that NanoNcS20 has a promising application in fluorescence imaging.Zebrafish infected with E.coli after tail injury were treated with NanoNcS20,the number of E.coli was reduced to 4% after laser irradiation for 30 minutes,indicating that NanoNcS20 has a good prospect of PDT/PTT antibacterial application.(2)Preparation,characterization and PDT/PTT antibacterial research of Zn Pc@Gly.Glycine-coated tetraamino zinc phthalocyanine carbon quantum dots(Zn Pc@Gly)were synthesized by microwave method,and characterized by UV absorption spectroscopy,fluorescence emission spectroscopy,and thermogravimetric analysis.TEM images show that Zn Pc@Gly is a spherical nano particle with an average diameter of 2 nm and a singlet oxygen yield of 8.6%under 660 nm(0.5W/cm~2)laser irradiation.Under the irradiation of 808 nm(0.5W/cm~2)laser,the photothermal conversion efficiency of Zn Pc@Gly is 8.8%.The photodynamic and photothermal activities of Zn Pc@Gly in vitro were studied.The mixture of bacteria and drugs was irradiated for 30 minutes by irradiation at 660 and 808 nm.When the concentration of Zn Pc@Gly was 0.08μM,the inhibition rate of E.coli and S.aureus were 100%and99.05%.indicating that it had good antibacterial activity of PDT/PTT.