Experimental Study On Photodynamic-Photothermal Synergistic Treatment Of Tumor And Resistant Bacteria With Substituted Phthalocyanine Nanophotosensitizers

The photo-based therapeutic approaches have attracted tremendous attention in recent years especially in treatment and management of tumors.Photodynamic and photothermal are two major therapeutic modalities which are being applied in clinical therapy.The development of nanomaterials for photodynamic combined photothermal therapy has gained significant attention for its treatment efficacy.Imaging is helpful for precise treatment,thus improving the effect of photo-based therapeutic.In this paper,1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000](DSPE-PEG₂₀₀₀)was chosed as a drug carrier to load di-[4-(diphenylamino)-1-diphenic acid-1-carbobenzoxy-4-(1,1,1,3,3,3-hexafluoropropane-4-phenoxy)silicon(IV)phthalocyanine(TPA-FBPA-SiPc)to prepared the polymeric nanoparticles(DSPE@TPA-FBPA-SiPc)usiedco-solventmethod.DSPE@TPA-FBPA-SiPc can be effectively uptake by MCF-7 breast cancer cells and targeted to the lysosomes.After being irradiation at 671 nm laser,DSPE@TPA-FBPA-SiPc mediated PDT to generate reactive oxygen species in cells and induce cells death through apoptosis pathway.Mesoporous silica@Gold nanorod(AuNR@SiO₂-NH₂)was prepared by seed growth method.TPA-FBPA-SiPc@AuNR@SiO₂-NH₂nanocomposite system was obtained by loading TPA-FBPA-SiPc on AuNR@SiO₂-NH₂via mesoporous silica pore adsorption.The morphology and composition of TPA-FBPA-SiPc@AuNR@SiO₂-NH₂were characterized by TEM,DLS and EDS.TPA-FBPA-SiPc@AuNR@SiO₂-NH₂showed core-shell stucture with uniform size with well dispersion.It exhibited strong fluorescenceandhighsingletoxygenproductionability.TPA-FBPA-SiPc@AuNR@SiO₂-NH₂exhibit high photothermal conversion efficiency and laser controlled release properties.TPA-FBPA-SiPc@AuNR@SiO₂-NH₂can be uptaked by MCF-7 breast cancer cells and specifically targeted to mitochondria.After being irradiation by 671 nm laser,a large amount of ROS was produced in the cells.The TPA-FBPA-SiPc@AuNR@SiO₂-NH₂mdediated l photothermal therapy(PTT)synergitic photodynamic therapy(PDT)to kill the breast cancer cells were better than by PDT or PTT alone.TPA-FBPA-SiPc@AuNR@SiO₂-NH₂mediated PTT synergitic PDT efficacy wasconfirmedbyCCK-8andflowcytometrymethod.TPA-FBPA-SiPc@AuNR@SiO₂-NH₂is a kind of aggregation induced phthalocyanine nanophotosensitizer which has a very promising application in the treatment tumor by PDT synergitic PTT under the guidance of fluorescence imaging.Tet-SiPc@AuNR@SiO₂-NH₂nanocomposite system was synthesized.Tet-SiPc@AuNR@SiO₂-NH₂is a core-shell structure with uniform size with well dispersion.Tet-SiPc could be released from Tet-SiPc@AuNR@SiO₂-NH₂upon irradiation,And upon irradiation with 671 nm laser,Tet-SiPc@AuNR@SiO₂-NH₂can produce singlet oxygen efficiently.Taking E.coli DH5?was used as the model bacteria,Tet-SiPc@AuNR@SiO₂-NH₂exhibited pohotdynamic killing effect on both common bacteria and resistant bacteria,and the inhibition rates are above 99%.Tet-SiPc@AuNR@SiO₂-NH₂mediated the PDT and PTT synergitic effect could kill the the common bacteria and resistant bacteria through destroy the DNA and protein of bacteria.These results show that Tet-SiPc@AuNR@SiO₂-NH₂have excellent antibacterial properties for resistant bacteria.