Deciphering Directional Active Growth Of Neural Cells By The Induction Of Extremely Low Frequency Electromagnetic Field

The importance of the nervous system in mammals is self-evident.However,nerve cells are often unable to repair themselves effectively after physical or endogenous damage.The whole cell shows atrophy and apoptosis,which seriously affects the function and integrity of the whole nervous system.Nerve cells cannot be effectively repaired and activated,and there is no more thorough clinical treatment for related diseases.At present,many methods including stem cell regulation and drug intervention can be used to induce the differentiation of nerve cells.However,these induction methods often have disadvantages such as high cost,large side effects,difficult evaluation of potency and complex operation.The main conclusions of this article:Magnetic field with specific frequency and intensity can promote cell growth and differentiation.However,there are few reports on the induced contrast effect of electromagnetic fields on cells under different physiological states.In the neurodegenerative cell model,cells are often in a state of high oxidative stress and nutrient deficiency,and their metabolic characteristics are significantly different from those of normal nerve cells.Therefore,this paper mainly studies the use of extremely low frequency electromagnetic waves(1mT,50Hz)to induce cells of different metabolic states,and make them receive extremely low frequency electromagnetic field stimulation under different physiological conditions(normal,hungry),to observe the effects of extremely low frequency electromagnetic field exposure on SH-SY5Y cells.The growth and differentiation model of SH-SY5Y cells promoted by extremely low frequency electromagnetic field was established.Secondly,patch clamp technique was used to detect the changes of cell membrane potential after extremely low frequency electromagnetic field stimulation,which demonstrated the difference of induction of electromagnetic field on nerve cells in different physiological states.(1)Growth promotion effect of magnetic field on SH-SY5Y cells:cells were exposed to extremely low frequency electromagnetic field for 1 hour,4 hours and 24 hours respectively.With the extension of cell exposure time,cell activity would increase accordingly.After 4 hours of induction,the activity of nerve cells had been significantly improved.(2)The induction effect of magnetic field on the proliferation of two kinds of cells:the induction of extremely low frequency electromagnetic wave can promote the metabolism of nutrients in nerve cells,and then promote the physiological activities of cells.However,for nerve cells with metabolic disorders,this induction effect can maintain the physiological state of cells in a short period of time,but can’t further promote the growth and proliferation of cells.(3)The intrinsic principle of magnetic field induction and proliferation and differentiation:cell membrane potential,mitochondria and specific growth-related regulatory proteins are closely related to electromagnetic wave induction,which proves that electromagnetic wave can induce and regulate the growth and differentiation of nerve cells through specific cell signal transduction pathways.