| Objective:1.To investigate the expression of DAMPs,serum S100,CRP,SAA and UA in chronic non-communicable diseases including type 2 diabetes,H-type hypertension,Alzheimer's,Parkinson's and healthy people of different age and BMI groups,and to explore the expression of DAMPs in NCD and its relationship with age and BMI in healthy people,to evaluate its feasibility as serological markers of NCD and aging.2.To investigate the expression of IFN-?,TNF-?,IL-4,IL-6,IL-10,IL-17secreted by Th1,Th2,Th17 in NCD and healthy people,and to explore the relationship between DAMPs and cytokines.By analyzing the immune status characteristics of NCD,to provides new ideas for the prevention and treatment of age-related chronic diseases and delaying aging.Methods:1.A total of 182 patients with NCD diagnosed in the Second People's Hospital of Hunan province from January 2019 to December 2020 were collected,including 60 patients with type 2 diabetes,44 patients with H-type hypertension,21patients with Alzheimer's,57 patients with Parkinson's,and 69 healthy controls.A total of 358 healthy people were collected from the Health Physical Examination Center of Second People's Hospital of Hunan province.1)According to the age interval of 20years,they were divided into three groups:21 to 40 years old group(148),41 to 60years old group(106),61 to 80 years old group(104).2)BMI was calculated according to height and weight(BMI=weight÷height~2).According to the Health Industry Standard of the People's Republic of China-Weight Determination for Adults(standard No.WS/T428-2013),the boundary is BMI=24kg/m~2,BMI<24kg/m~2 were classified as non-overweight(218),and BMI?24kg/m~2 were classified as overweight(140).The level of serum S100,CRP,SAA and UA were detected by electrochemiluminescence,latex immunoturbidimetry,latex-enhanced immunoturbidimetry and uricase-peroxidase assay.2.One hundred and one patients with NCD were included in the study,including 30 patients with type 2 diabetes,27 patients with H-type hypertension,21patients with Alzheimer's disease,23 patients with Parkinson's disease,and 23 healthy controls.87 healthy people were included in the physical examination center of the hospital,1)Also divided by age group:21?40 years old group(29),41?60 years old group(31),61?80 years old group(27).2)According to weight,healthy people were divided into two groups:non-overweight(44)and overweight(43).The expression levels of IFN-?,TNF-?,IL-4,IL-6,IL-10 and IL-17 were detected by flow cytometry.Results:1.1)The levels of serum CRP,SAA and UA in type 2 diabetes group and H-type hypertension group were higher than those in healthy group,and the differences were statistically significant(P=0.001,P<0.001,P=0.005;P=0.037,P=0.042,P<0.001),and there was no significant difference in serum S100 levels(P>0.05);Only The level of serum SAA in the Alzheimer's group and Parkinson's group was higher than that of healthy group,and the difference was statistically significant(P<0.001;P<0.001).There were no significant differences in serum S100,CRP and UA levels(P>0.05).2)Serum SAA,Hb A1c,GLU were positively correlated with CRP in type 2diabetes group(P=0.002,P=0.008,P=0.009),and serum S100,CRP were positively correlated with SAA in H-type hypertension(P=0.035,P=0.003),serum CRP was positively correlated with SAA in the Alzheimer's group(P=0.002),and serum S100,SAA were positively correlated with CRP in Parkinson's group(P=0.014,P<0.001),but there was no significant correlation between other indexes(P>0.05).3)There were statistically significant differences in serum S100,CRP,SAA and UA levels among the three age groups in healthy people(P=0.015,P<0.001,P<0.001,P=0.003).The levels of serum CRP and SAA in the 41 to 60 years old group were significantly higher than those in the 21 to 40 years old group(P<0.001,P=0.018),The levels of serum S100,CRP,SAA and UA in 61-80 years old group were significantly higher than those in 21-40 years old group(P=0.025,P<0.001,P<0.001,P=0.008);The serum levels of S100,SAA and UA in 61-80 years old group were significantly higher than those in41-60 years old group(P=0.042,P=0.029,P=0.008).The serum CRP levels in 61-80 years old group were higher than those in 41-60 years old group,but the difference was not statistically significant(P>0.05).The levels of serum S100,CRP,SAA and UA increased with age.The levels of serum CRP,SAA and UA in overweight were significantly higher than those in non-overweight(P<0.001,P<0.001,P<0.001),there was no significant difference serum S100(P>0.05).4)Age,BMI,GLU,TG,SAA were positively correlated with serum CRP in healthy subjects(P<0.001,P<0.001,P<0.001,P<0.001,P<0.001);Serum SAA was positively correlated with age and BMI(P<0.001,P<0.001);Serum UA was positively correlated with BMI and TG(P<0.001,P<0.001);Serum CRP and UA were negatively correlated with HDL-C(P<0.001,P<0.001).Logistic regression analysis showed that CRP,SAA and UA were risk factors for overweight population(P<0.05).2.1)The level of serum IL-17in type 2 diabetes group was higher than that of healthy control group(P=0.001),and there were no significant in serum IL-6,IL-10,IFN-?and IL-4(P>0.05);The levels of serum IL-6,IL-10,IFN-?and IL-17 in H-type hypertension group were higher than those in healthy control group(P<0.001,P=0.006,P=0.003,P<0.001),there was no significant difference in serum IL-4(P>0.05);The levels of serum IL-6,IL-10,IFN-?,IL-17 and IL-4 in the Alzheimer's group were higher than those in the healthy group(P=0.001,P=0.004,P=0.001,P<0.001,P<0.001);The level of serum IL-6 and IL-17 in Parkinson's group were significantly higher than those in healthy group(P=0.005,P=0.011),The level of serum IL-10,IFN-?and IL-4 were not significantly different(P>0.05).2)Serum SAA was positively correlated with IL-17(P=0.024),and serum UA was positively with IL-17 and IFN-?in type 2 diabetes group(P=0.028,P=0.014).There was a positive correlation between serum UA and IFN-?in H-type hypertension group(P=0.007).There was a positive correlation between serum CRP and IL-6 in Alzheimer's group(P=0.018).In Parkinson's group,serum CRP was positively correlated with IL-6 and IL-17(P=0.008,P=0.018),and serum SAA was positively correlated with IL-10(P=0.008).3)There were significant differences in serum IL-6 among all age group(P<0.001),serum IL-6 increased with age;There was a significant difference in serum IL-4 among all age groups(P=0.011).There was little change in serum IL-4 under 60 years old but decreased after 60 years old.There was no significant change trend in the other indexes in each age group.There was no significant difference in serum cytokine levels among different BMI groups(P>0.05).4)Serum IL-6 was positively correlated with CRP,SAA and UA in healthy people(P<0.001,P<0.001,P=0.001),and serum IL-6 was positively correlated with age(P<0.001).Conclusion:1.DAMPs S100,CRP,SAA and UA in NCD increased to a certain extent,and showed an upward trend with the increase of age and BMI.Therefore,the role of DAMPs as markers of NCD and inflamm-aging is worth further study.2.The types of immune activation of various chronic diseases are different:only the level of cytokine IL-17 was increased in type 2 diabetes;the levels of IFN-?,IL-6,IL-10,and IL-17 were increased in H-type hypertension,the levels of IFN-?,IL-4,IL-6,IL-10 and IL-17 were all increased in Alzheimer's,and Th1,Th2 and Th17 cells were all activated;the levels of IL-6 and IL-17 were increased in Parkinson's,and Th2 and Th17 cells were activated,but no evidence of Th1 cell activation was found;3.Chronic inflammatory response mediated by DAMPs is involved in the occurrence and development of age-related diseases and regulates chronic inflammation of the body.This study provides new ideas for the prevention and treatment of NCD and delaying aging. |
PDF Full Text Request