Association Of BDNF/TrkB Signaling Pathway Gene Polymorphism With Phenotypic Sensitivity Of Aerobic Endurance After High Intensity Interval Training

Research objectives:In this paper,by studying the association between BDNF/Trk B signaling pathway gene polymorphisms and changes in aerobic endurance indicators after high-intensity interval training（HIIT）,this paper explores the genetic markers of HIIT aerobic endurance phenotypic sensitivity,with a view to formulating populations with different genetic characteristics Provide basis for precise exercise and fitness guidance program.Research objects and methods:In this study,220 healthy college students of the Han were selected from 4 colleges and universities of Jiangxi Normal University,Inner Mongolia Normal University,Anqing Normal University,and Lanzhou City University.All of them had irregular exercise history and had signed informed consent.The subjects underwent HIIT intervention for 12 weeks,training 3 times a week,and tested their maximum oxygen uptake（VO₂max）,ventilatory threshold（VT）,energy saving（EE）and 800m/1000m running performance before and after the exercise intervention.In addition,DNA was extracted from venous blood,and the BDNF gene rs6265,rs11030101,and Trk B gene rs1187323 were genotyped using the whole gene sequencing method.Results:1.The rs1187323 polymorphism of Trk B gene is associated with the changes in800-meter running performance of girls.The improvement of running performance of people with CC genotype is significantly greater than that of people with AA genotype.2.The rs6265 polymorphism of the BDNF gene is associated with changes in the minute ventilation（VE）and maximum heart rate（HRmax）of girls under VO₂max.Among them,the increase in VE of TT genotypes was significantly greater than that of CC and CT genotypes,and TT genotypes The increase in HRmax of patients with CT genotype was significantly greater.3.The rs11030101 polymorphism of the BDNF gene is associated with changes in VO₂max（L/min,ml/min/kg）of girls,and the increase in VO₂max（L/min,ml/min/kg）of TT genotypes is significantly less than that of AA And AT genotype.4.The rs11030101 polymorphism of the BDNF gene is associated with changes in the oxygen uptake（VO₂（L/min））of girls under VT.The increase in VO₂（L/min）of the AA genotype is significantly greater than that of the AT genotype.5.The rs6265 polymorphism of the BDNF gene is associated with changes in VO₂（L/min）and heart rate（HR）of girls under EE.Among them,the increase inVO₂（L/min）of the CC genotype is significantly greater than that of the TT genotype,CT The reduction of HRmax of genotypes was significantly smaller than that of CC genotypes.6.The rs11030101 polymorphism of the BDNF gene is associated with changes in male HR and female VE under EE.The reduction in HR of males with AT and TT genotypes was significantly greater than that of AA genotypes;the VE of females with AT and TT genotypes increased The amplitude was significantly greater than those of AA genotype.7.The rs1187323 polymorphism of Trk B gene is related to the change of VO₂（ml/min/kg）in boys with EE.Among them,the increase of VO₂（ml/min/kg）of the CC genotype is significantly greater than that of the AC genotype.Research Conclusions:1.The rs1187323 polymorphism of Trk B gene is associated with the changes in800-meter running performance of girls after HIIT,which suggests that the rs1187323 polymorphism of Trk B gene can be used as a genetic marker for the sensitivity of the 800-meter running performance of Chinese Han female college students after HIIT training.2.The rs6265 polymorphism of the BDNF gene is associated with the changes in VE and HRmax under the VO₂max test of girls after HIIT,and the rs11030101polymorphism of the BDNF gene is associated with the changes in VO₂max（L/min,ml/min/kg）of the girls after HIIT,which suggests The rs6265 and rs11030101 polymorphisms of BDNF gene can be used as genetic markers for the sensitivity of VO₂max related indexes after HIIT in Chinese Han female college students.3.The rs11030101 polymorphism of the BDNF gene is associated with the change of VO₂（L/min）in female VT after HIIT,which suggests that the rs11030101polymorphism of the BDNF gene can be used as a genetic marker for the VT sensitivity of female college students in Han Chinese.4.The rs6265 polymorphism of the BDNF gene is associated with the changes of VO₂（L/min）and HR in girls after HIIT.The rs11030101 polymorphism of the BDNF gene is associated with the changes of HR in boys after HIIT.The Trk B gene rs1187323 polymorphism is associated with the change of VO₂（ml/min/kg）of male EE after HIIT,which suggests that the BDNF gene rs6265 polymorphism can be used as a genetic marker for the sensitivity of EE-related indicators after HIIT in Chinese Han female college students,and the BDNF gene rs11030101 polymorphis m and Trk B gene rs1187323 polymorphism can be used as genetic markers for the sensitivity of related indicators in EE after HIIT in Chinese Han male college students.