| Research Objective:To explore whether combined use of L-carnitine,pantothenic acid and coenzyme Q10 can improve exercise-induced-fatigue in mice,the best combination of combined use and the mechanism.Research Methods:(1)Experimental subjects:100 ICR male mice,aged 6-8 weeks,with the body weight of 18-22g.(2)Experimental groups:A total of 100 male ICR mice were randomly divided into 10 groups with 10 mice in each group.The orthogonal design was used to determine the dose and exercise.L-camitine,pantothenic acid and coenzyme Q10 were divided into three dose levels:no use,low dose and high dose.Swimming training was the fourth influencing factor,which was divided into two levels:training and no training.There were 10 groups in total including exercise control group,with 10 mice in each group.Mice in exercise group swam with 5%weight for 1 hour per day,6 days per week for 6 weeks.Nutritional factors were administered by oral gavage 1 hour before exercise.(3)Detect indicators:At the end of the fifth week,an exhaustive exercise was conducted and the exhaustive time was recorded.Continue the exercise for another week,and samples were taken 24 hours after the last exercise at the end of the sixth week.The serum urea nitrogen(BUN),the ratio of testosterone to cortisol(T/C),the activity of superoxide dismutase(SOD)in serum and skeletal muscle tissue,and the content of malondialdehyde(MDA)were determined.The mRNA expression levels of Kelch-like ech-related protein 1(Keap1),nuclear factor E2-related factor 2(Nrf2)and heme oxygenase(HO-1)in skeletal muscle tissue of mice were detected by RT-PCR.Results:(1)There were significant differences in load-bearing swimming exhaustion time of mice among all groups.The effect of weight-bearing swimming exhaustion time on mice in order of importance is exercise>pantothenic acid>coenzyme Q10>L-carnitine,and the longest exhaustion time was A1B3C3D2 combination(exercise combined with nutritional factor group,EM group).(2)The level of BUN in exercise control group(E)was significantly higher than that in blank control group(NC)(p<0.05),and the level of BUN in exercise combined with nutritional factor supplementation group(EM)was significantly lower than that in E group(p<0.01).Serum T/C in group E was significantly decreased compared with that in NC group(p<0.01),and T/C in group EM was significantly increased compared with that in group E(p<0.01).(3)The SOD levels in serum and muscle tissue of mice in group E were significantly lower than those in group NC(p<0.01,p<0.01),and those in serum and muscle tissue of mice in group EM were significantly higher than those in group E(p<0.05,p<0.05).The MDA levels in serum and muscle tissue of mice in group E were significantly higher than those in group NC(p<0.05,p<0.05),and the MDA levels in serum and muscle tissue of mice in group EM were significantly lower than those in group E(p<0.05,p<0.01).(4)Compared with NC group,the expression level of Keap1 mRNA in skeletal muscle of mice in group E was significantly decreased(p<0.01),and the expression level of Keapl mRNA in group EM was significantly higher than that in group E.The mRNA expression level of Nrf2 in skeletal muscle in group E was significantly decreased compared with that in group NC(p<0.01),and the mRNA expression level of Nrf2 in skeletal muscle in group EM was significantly increased compared with that in group E(p<0.05).Compared with NC group,the mRNA expression level of skeletal muscle HO-1 in group E was significantly decreased(p<0.01),while the mRNA expression level of skeletal muscle HO-1 in group EM was significantly increased(p<0.05).Research Conclusions:(1)The effect of exogenous nutritional factors on prostretching the exhaustive time of weight-bearing swimming in mice was in descending order:pantothenic acid>coenzyme Q10>L-carnitine.(2)The combination of pantothenic acid and coenzyme Q10 can reduce the level of blood urea nitrogen and increase the serum T/C ratio in mice;The content of SOD in serum and skeletal muscle was increased,and the level of lipid peroxide MDA was decreased.The exhaustion time of weight-bearing swimming was prolonged.(3)The combination of pantothenic acid and coenzyme Q10 can regulate the Keap1/Nrf2/HO-1 signaling pathway and alleviate oxidative stress. |
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